Abstract
When the Human Genome Mapping Project began in earnest, the allocation of large funding and the opening of dedicated research facilities promised much for the rapid improvement of DNA sequencing methods and, as a consequence, sequencing rates and throughput. Today, several years later, some novel approaches to the problem are being developed, such as sequencing by hybridization, and sequence detection using mass spectrometry and biochips, but these are far from in general use.
Similar content being viewed by others
References
Messing, J. and Bankier, A. T. (1989) The use of single-stranded DNA phage in DNA sequencing, in Nucleic Acids Sequencing: A Practical Approach (Howe C. J. and Waed E. S., eds.), IRL Press, Oxford, pp. 1–36.
Hong, G. F. (1982) A systematic DNA sequencing strategy. J. Mol. Biol. 158, 539–549.
Henikoff, S. (1984) Unidirectional digestion with exonuclease III cre-ates targeted breakpoints for DNA sequencing. Gene 28, 351–359.
Bankier, A. T., Weston, K. M., and Barrell, B. G. (1987) Random cloning and sequencing by the M13/dideoxynucleotide chain termi-nation method, in Methods in Enzymology, vol. 155 (Wu, R. ed.), Academic Press, London, pp. 51–93.
Smith, V., Craxton, M., Bankier, A. T., Brown, C. M., Rawlinson, W. D., Chee, M. S., and Barrell, B. G. (1993) Preparation and fluores-cent sequencing of M13 clones: microtitre methods, in Methods in Enzymology, vol. 218 (Wu, R. ed.), Academic Press, London, pp. 155–173.
Oefner, P. J., Hunicke-Smith, S. P., Chiang, L., Dietrich, F., Mulligan, J., and Davis, R. W. (1996) Efficient random subcloning of DNA sheared in a recirculating point-sink flow system. Nucleic Acids Res. 24, 3879–3886.
Anderson, S. (1981) Shotgun DNA sequencing using cloned DNAse 1-generated fragments. Nucleic Acids Res. 9, 3015.
Deininger, P. L. (1983) Random subcloning of sonicated DNA: Application to shotgun DNA sequence analysis. Anal. Biochem. 129, 216.
Sambrook, J., Frisch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc.
About this protocol
Cite this protocol
Bankier, A.T. (2001). Shotgun DNA Sequencing. In: Graham, C.A., Hill, A.J.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 167. Humana Press. https://doi.org/10.1385/1-59259-113-2:089
Download citation
DOI: https://doi.org/10.1385/1-59259-113-2:089
Publisher Name: Humana Press
Print ISBN: 978-0-89603-716-8
Online ISBN: 978-1-59259-113-8
eBook Packages: Springer Protocols