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Adoptive Immunotherapy

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Burkhard Ludewig

    1. Research Department, Kantonsspital St. Gallen, Switzerland

  2. Matthias W. Hoffmann

    1. Department of Abdominal and Transplant Surgery, Hannover Medical School, Hannover, Germany

Part of the book series: Methods in Molecular Medicine (MIMM, volume 109)

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About this book

An authoritative collection of optimal techniques for producing and characterizing the immunologically active cells and effector molecules now gaining wide use in the clinical treatment of patients. Taking advantage of the latest technologies, the authors present readily reproducible experimental protocols for the study of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and clinical applications and on the progress of selected approaches in clinical trials. Additional chapters cover the molecular definition of target antigens, mathematical modeling approaches to immunotherapy, and the utilization of regulatory T cells. The protocols make it possible to study the adoptive transfer of tailored antigen-specific immune cells and to improve the clinical application of adoptive immunotherapy.

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Exploiting Dendritic Cells for Active Immunotherapy of Cancer and Chronic Infection

    • David O’Neill, Nina Bhardwaj
    Pages 1-18

  3. A Mathematical Approach for Optimizing Dendritic Cell-Based Immunotherapy

    • Gennady Bocharov, Neville J. Ford, Burkhard Ludewig
    Pages 19-33

  4. Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres

    • Ying Waeckerle-Men, Bruno Gander, Marcus Groettrup
    Pages 35-46

  5. RNA Transfection of Dendritic Cells

    • Frank Grünebach, Martin R. Müller, Peter Brossart
    Pages 47-54

  6. Isolation and Generation of Clinical-Grade Dendritic Cells Using the CliniMACS System

    • John D. M. Campbell, Christoph Piechaczek, Gregor Winkels, Edith Schwamborn, Daniela Micheli, Sonja Hennemann et al.
    Pages 55-70

  7. Generation of Clinical-Grade Monocyte-Derived Dendritic Cells Using the CliniMACS System

    • Thomas Putz, Hubert Gander, Reinhold Ramoner, Claudia Zelle-Rieser, Andrea Rahm, Walter Nussbaumer et al.
    Pages 71-81

  8. Adenoviral Transduction of Dendritic Cells

    • Rienk Offringa, Kitty Kwappenberg, Martijn Rabelink, Delphine Rea, Rob Hoeben
    Pages 83-95

  9. Generation of Autologous Peptide- and Protein-Pulsed Dendritic Cells for Patient-Specific Immunotherapy

    • David O’Neill, Nina Bhardwaj
    Pages 97-112

  10. Phenotypical and Functional Characterization of Clinical-Grade Dendritic Cells

    • I. Jolanda, M. de Vries, Gosse J. Adema, Cornelis J. A. Punt, Carl G. Figdor
    Pages 113-125

  11. Dendritic Cells in Clinical Trials for Multiple Myeloma

    • Volker L, Reichardt and Peter Brossart
    Pages 127-135

  12. Identification of Tumor-Associated Autoantigens With SEREX

    • Özlem Türeci, Dirk Usener, Sandra Schneider, Ugur Sahin
    Pages 137-154

  13. Application of Proteomics and Protein Analysis for Biomarker and Target Finding for Immunotherapy

    • Petra Weingarten, Petra Lutter, Andreas Wattenberg, Martin Blueggel, Sonja Bailey, Joachim Klose et al.
    Pages 155-173

  14. Isolation and Expansion of Tumor-Reactive Cytotoxic T-Cell Clones for Adoptive Immunotherapy

    • Helga Bernhard, Burkhard Schmidt, Dirk H. Busch, Christian Peschel
    Pages 175-184

  15. Tracking Adoptively Transferred Antigen-Specific T-Cells With Peptide/MHC Multimers

    • Norbert Meidenbauer, Andreas Mackensen
    Pages 185-200

  16. Gene Transfer of MHC-Restricted Receptors

    • Helmut W. H. G. Kessels, Monika C. Wolkers, Ton N. M. Schumacher
    Pages 201-213

  17. Immunotherapy With CTL Restricted by Nonself MHC

    • Liquan Gao, Anne-Marie Downs, Hans J.
    Pages 215-228

  18. Designing TCR for Cancer Immunotherapy

    • Ralf-Holger Voss, Jürgen Kuball, Matthias Theobald
    Pages 229-256

  19. Isolation and Expansion of Tumor-Specific CD4+T-Cells by Means of Cytokine Secretion

    • Christian Becker
    Pages 257-264

  20. Methods for the Ex Vivo Characterization of Human CD8+T Subsets Based on Gene Expression and Replicative History Analysis

    • Nathalie Rufer, Patrick Reichenbach, Pedro Romero
    Pages 265-283
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Reviews

"...a comprehensive and authoritative collection of currently used protocols for adoptive immunotherapy in both pre-clinical and clinical settings." - Immunology News

"...will be not only useful for basic researchers but also clinical scientists aiming to translate basic research to a clinical setting." - Immunology and Cell Biology

Editors and Affiliations

  • Research Department, Kantonsspital St. Gallen, Switzerland

    Burkhard Ludewig

  • Department of Abdominal and Transplant Surgery, Hannover Medical School, Hannover, Germany

    Matthias W. Hoffmann

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Bibliographic Information

  • Book Title: Adoptive Immunotherapy

  • Book Subtitle: Methods and Protocols

  • Editors: Burkhard Ludewig, Matthias W. Hoffmann

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592598625

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-406-7Published: 30 November 2004

  • Softcover ISBN: 978-1-61737-546-0Published: 09 November 2010

  • eBook ISBN: 978-1-59259-862-5Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XVI, 500

  • Topics: Immunology

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