Home > Archive>Volume 11, Issue 3, 2018 >354-362. DOI:10.18240/ijo.2018.03.02
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Scleral ultrastructure and biomechanical changes in rabbits after negative lens application
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Corresponding Author:

Hao Zhou. Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, No.83 Fenyang Road, Shanghai 200031, China. zheent@163.com

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Supported by the Science and Technology Commission of Shanghai Municipality (No.134119a5100).

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    Abstract:

    AIM: To address the microstructure and biomechanical changes of the sclera of rabbits after negative lens application by spectacle frame apparatus. METHODS: Five New Zealand rabbits of seven weeks post-natal were treated with -8 D lens monocularly over the course of two weeks. Refractive errors and axial length (AXL) were measured at the 1st, 7th and 14th days of the induction period. Ultrastructure of sclera was determined with electron microscopy. Biomechanical properties were tested by an Instron 5565 universal testing machine. RESULTS: Lens-induced (LI) eyes elongated more rapidly compared with fellow eyes with AXL values of 15.56±0.14 and 15.21±0.14 mm (P<0.01). Fibril diameter was significantly smaller in the LI eyes compared with control ones in the inner, middle, and outer layers (inner layer, 63.533 vs 76.467 nm; middle layer, 92.647 vs 123.984 nm; outer layer, 86.999 vs 134.257 nm, P<0.01, respectively). In comparison with control eyes, macrophage-like cells that engulfed fibroblasts, dilated endoplasmic reticulum, and vacuoles in fibroblasts were observed in the inner and middle stroma in the LI eyes. Ultimate stress and Young’s modulus were lower in the LI eyes compared with those in the control eyes. CONCLUSION: Negative lens application alters eye growth, and results in axial elongation with changes in scleral ultrastructural and mechanical properties.

    Reference
    1 Morgan IG, Ashby RS, Nickla DL. Form deprivation and lens-induced myopia: are they different? Ophthal Physl Opt 2013;33(3):355-361.
    2 Rada JA, Shelton S, Norton TT. The sclera and myopia. Exp Eye Res 2006;82(2):185-200.
    3 Grytz R, Siegwart JJ Jr. Changing material properties of the tree shrew sclera during minus lens compensation and recovery. Invest Ophthalmol Vis Sci 2015;56(3):2065-2078.
    4 Lin Z, Chen X, Ge J, Cui D, Wu J, Tang F, Tan J, Zhong X, Gao Q. Effects of direct intravitreal dopamine injection on sclera and retina in form-deprived myopic rabbits. J Ocul Pharmacol Ther 2008;24(6):543-550.
    5 McBrien NA, Jobling AI, Gentle A. Biomechanics of the sclera in myopia: extracellular and cellular factors. Optom Vis Sci 2009;86(1):23-30.
    6 Murienne BJ, Jefferys JL, Quigley HA, Nguyen TD. The effects of glycosaminoglycan degradation on the mechanical behavior of the posterior porcine sclera. Acta Biomater 2015;12:195-206.
    7 Liu S, Li S, Wang B, Lin X, Wu Y, Liu H, Qu X, Dai J, Zhou X, Zhou H. Scleral cross-linking using riboflavin UVA irradiation for the prevention of myopia progression in a guinea pig model: Blocked axial extension and altered scleral microstructure. PLoS One 2016;11(11):e165792.
    8 Siegwart JJ Jr, Strang CE. Selective modulation of scleral proteoglycan mRNA levels during minus lens compensation and recovery. Mol Vis 2007;13:1878-1886.
    9 Moring AG, Baker JR, Norton TT. Modulation of glycosaminoglycan levels in tree shrew sclera during lens-induced myopia development and recovery. Invest Ophthalmol Vis Sci 2007;48(7):2947-2956.
    10 Gao H, Frost MR Jr, Siegwart JT, Norton TT. Patterns of mRNA and protein expression during minus-lens compensation and recovery in tree shrew sclera. Mol Vis 2011;17:903-919.
    11 Hammond DS, Wallman J, Wildsoet CF. Dynamics of active emmetropisation in young chicks--influence of sign and magnitude of imposed defocus. Ophthalmic Physiol Opt 2013;33(3):215-226.
    12 Benavente-Perez A, Nour A, Troilo D. The effect of simultaneous negative and positive defocus on eye growth and development of refractive state in marmosets. Invest Ophthalmol Vis Sci 2012;53(10):6479-6487.
    13 Smith ER 3rd, Hung LF, Arumugam B, Huang J. Negative lens-induced myopia in infant monkeys: effects of high ambient lighting. Invest Ophthalmol Vis Sci 2013;54(4):2959-2969.
    14 Prince JH. The rabbit in eye research. Springfield, Ill.: C.C. Thomas; 1964.
    15 Ahn SJ, Hong HK, Na YM, Park SJ, Ahn J, Oh J, Chung JY, Park KH, Woo SJ. Use of rabbit eyes in pharmacokinetic studies of intraocular drugs. J Vis Exp 2016;(113).
    16 Maurice DM, Mushin AS. Production of myopia in rabbits by raised body-temperature and increased intraocular pressure. Lancet 1966;2(7474):1160-1162.
    17 Verolino M, Nastri G, Sellitti L, Costagliola C. Axial length increase in lid-sutured rabbits. Surv Ophthalmol 1999;44(Suppl 1):S103-S108.
    18 Dotan A, Kremer I, Gal-Or O, Livnat T, Zigler A, Bourla D, Weinberger D. Scleral cross-linking using riboflavin and ultraviolet-A radiation for prevention of axial myopia in a rabbit model. J Vis Exp 2016;(110):e53201.
    19 Nie HH, Huo LJ, Yang X, Gao ZY, Zeng JW, Trier K, Cui DM. Effects of 7-methylxanthine on form-deprivation myopia in pigmented rabbits. Int J Ophthalmol 2012;5(2):133-137.
    20 Bryant MR, Kampmeier J, Er H, Kasetsuwan N, Sanchez-DiMartino D, Shah SS, McDonnell PJ. PRK-induced anisometropia in the rabbit as a model of myopia. Graefes Arch Clin Exp Ophthalmol 1999;237(2):161-165.
    21 Tokoro T. Experimental myopia in rabbits. Invest Ophthalmol 1970; 9(12):926-934.
    22 Funata M, Tokoro T. Scleral change in experimentally myopic monkeys. Graefes Arch Clin Exp Ophthalmol 1990;228(2):174-179.
    23 Wollensak G, Iomdina E. Crosslinking of scleral collagen in the rabbit using glyceraldehyde. J Cataract Refract Surg 2008;34(4):651-656.
    24 Sivak JG. The role of the lens in refractive development of the eye: animal models of ametropia. Exp Eye Res 2008;87(1):3-8.
    25 Norton TT, McBrien NA. Normal development of refractive state and ocular component dimensions in the tree shrew (Tupaia belangeri). Vision Res 1992;32(5):833-842.
    26 Girard MJ, Dahlmann-Noor A, Rayapureddi S, Bechara JA, Bertin BM, Jones H, Albon J, Khaw PT, Ethier CR. Quantitative mapping of scleral fiber orientation in normal rat eyes. Invest Ophthalmol Vis Sci 2011;52(13):9684-9693.
    27 Christiansen DL, Huang EK, Silver FH. Assembly of type I collagen: fusion of fibril subunits and the influence of fibril diameter on mechanical properties. Matrix Biol 2000;19(5):409-420.
    28 Parry DA. The molecular and fibrillar structure of collagen and its relationship to the mechanical-properties of connective-tissue. Biophys Chem 1988;29(1-2):195-209.
    29 Gentle A, Liu Y, Martin JE, Conti GL, McBrien NA. Collagen gene expression and the altered accumulation of scleral collagen during the development of high myopia. J Biol Chem 2003;278(19):16587-16594.
    30 Rada JA, Nickla DL, Troilo D. Decreased proteoglycan synthesis associated with form deprivation myopia in mature primate eyes. Invest Ophthalmol Vis Sci 2000;41(8):2050-2058.
    31 Long Q, Ye J, Li Y, Wang S, Jiang Y. C-Reactive protein and complement components in patients with pathological myopia. Optom Vis Sci 2013;90(5):501-506.
    32 Backhouse S, Phillips JR. Effect of induced myopia on scleral myofibroblasts and in vivo ocular biomechanical compliance in the guinea pig. Invest Ophthalmol Vis Sci 2010;51(12):6162-6171.
    33 Grytz R, Fazio MA, Girard MJ, Libertiaux V, Bruno L, Gardiner S, Girkin CA, Downs JC. Material properties of the posterior human sclera. J Mech Behav Biomed Mater 2014; 29: 602-617.
    34 Fazio MA, Grytz R, Bruno L, Girard MJ, Gardiner S, Girkin CA, Downs JC. Regional variations in mechanical strain in the posterior human sclera. Invest Ophthalmol Vis Sci 2012;53(9):5326-5333.
    35 Metlapally S, McBrien NA. The effect of positive lens defocus on ocular growth and emmetropization in the tree shrew. J Vis 2008; 8(3):1.1-12.
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Xiao Lin, Bing-Jie Wang, Yen-Chiao Wang,/et al.Scleral ultrastructure and biomechanical changes in rabbits after negative lens application. Int J Ophthalmol, 2018,11(3):354-362

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Publication History
  • Received:July 19,2017
  • Revised:January 15,2018
  • Online: March 18,2018
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