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Dectin-1 plays a prominent role in the immune response against the dermatophyte "Trichophyton rubrum" in murine model

Fábio S. Yoshikawa1* and Sandro R. De Almeida1
  • 1 Universidade de São Paulo, Department of Clinical and Toxicological Analyses - Faculty of Pharmaceutical Sciences, Brazil

Introduction: "Trichophyton rubrum" is the main agent of dermatophytosis in humans. However, little is known about the immunological mechanisms underlying these mycoses.
Dectin-1 is an immune receptor involved in responses to fungi. It can induce production of cytokines and shape the immune response, like IL-1β, a key cytokine for the induction of TH17 responses.
The aim of this study was to elucidate the role of Dectin-1 in the immune response against "T.rubrum".
Materials and Methods: Bone-marrow derived macrophages (BMMs) from C57BL/6 mice (wild-type and dectin-1-/-) were primed with LPS and incubated with "T.rubrum" conidia for 4, 6, 8 and 10 hours. The interaction was evaluated by optical microscopy and IL-1β levels were measured by ELISA.
Groups of 3 C57BL/6 mice (wild-type and dectin-1-/-) were infected with "T.rubrum" conidia intraperitoneally. Animals were maintained for 7 and 14 days before being euthanized. Liver and spleen were collected for determination of fungal burden and cytokine measurements (IL-1β, IFN-γ, IL-4).
Results: Wild-type BMMs phagocytosed conidia and secreted IL-1β in response to the pathogen, but were destroyed by the fungal growth. Dectin-1-/- counterparts, however, showed reduced phagocytose and cytokine production, but conidia were unable to become hyphae.
Dectin-1-/- mice could not control the fungal burden and showed higher production of IFN-γ and IL-4, but lower levels of IL-1β, than their wild-type correlates.
Conclusions: Results showed that Dectin-1 is important for phagocytose of "T.rubrum" conidia and production of IL-1β. "In vivo" results suggest that dectin-1 is necessary for infection control by induction of IL-1β.

Acknowledgements

Financial Support: FAPESP

References

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Keywords: dectin-1, Trichophyton rubrum, IL-1beta, Macrophages, dermatophyte

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Host-pathogen interactions

Citation: Yoshikawa FS and De Almeida SR (2013). Dectin-1 plays a prominent role in the immune response against the dermatophyte "Trichophyton rubrum" in murine model. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00059

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Received: 08 Mar 2013; Published Online: 22 Aug 2013.

* Correspondence: Mr. Fábio S Yoshikawa, Universidade de São Paulo, Department of Clinical and Toxicological Analyses - Faculty of Pharmaceutical Sciences, São Paulo, São Paulo, 05508-000, Brazil, faseiti@gmail.com