Research Article

In vitro Antibacterial Activity of Ethanolic Tanao Si Kan Dang RD1 (Cannabis sativa L.) Extracts Against Human Antibiotic-Resistant Bacteria

Nipaporn Armassa
Faculty of Agricultural Innovation and Technology, Rajamangala University of Technology Isan, Nakhon Ratchasima 30000, Thailand
LiveDNA: 66.43220

Duanpen Wongsorn
Faculty of Agricultural Innovation and Technology, Rajamangala University of Technology Isan, Nakhon Ratchasima 30000, Thailand
LiveDNA: 66.33972

Benya Saenmahayak
Faculty of Agricultural Innovation and Technology, Rajamangala University of Technology Isan, Nakhon Ratchasima 30000, Thailand

Somsak Rayan
Faculty of Natural Resources Rajamangala, University of Technology Isan, Sakon Nakhon Campus, Phang Khon Sub District, Phang Khon District, Sakon Nakhon, 47160, Thailand

Surachai Rattanasuk
Department of Science and Technology, Faculty of Liberal Arts and Science, Roi Et Rajabhat University, Selaphum, Roi Et 45102, Thailand
LiveDNA: 66.12978
ORCID: 0000-0002-8558-7538

Background and Objective: A new strain of cannabis, Cannabis sativa L. Tanao Si Kan Dang RD1, has been approved and registered by the Rajamangala University of Technology Isan, Thailand. The C. sativa is acknowledged for its medicinal properties which demonstrated various therapeutic properties, such as anti-cancer and antibacterial activities. This study aimed to investigate the antibacterial activity of ethanolic extracts from the stems and leaves of the Tanao Si Kan Dang RD1 strain against seven antibiotic-resistant bacteria. Materials and Methods: The primary antibacterial activity of ethanolic Tanao Si Kan Dang RD1 extracts were determined using the disc diffusion method, while the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined using the broth microdilution method. Results: The largest inhibition zone, measuring 12 mm, was observed in leaf extracts against Pseudomonas aeruginosa 101. The lowest MIC, at 0.78 mg/mL, was obtained from stem extracts against Stenotrophomonas maltophilia. The lowest MBCs, at 12.5 mg/mL, were observed in leaf extracts against Enterococcus faecalis, Acinetobacter baumannii, multidrug-resistant Klebsiella pneumoniae, Stenotrophomonas maltophilia and Pseudomonas aeruginosa 101 and stem extracts against Acinetobacter baumannii, multidrug-resistant Klebsiella pneumoniae, Stenotrophomonas maltophilia and Pseudomonas aeruginosa 101. Conclusion: This study presents a novel finding regarding the antibacterial activity of ethanolic extracts from the leaves and stems of Tanao Si Kan Dang RD1 against antibiotic-resistant bacteria. The potential application of these cannabis plant extracts in the development of antibiotics capable of combating antibiotic-resistant pathogenic bacteria represents a promising strategy to address a significant global health concern.

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Nipaporn Armassa, Duanpen Wongsorn, Benya Saenmahayak, Somsak Rayan and Surachai Rattanasuk, 2024. In vitro Antibacterial Activity of Ethanolic Tanao Si Kan Dang RD1 (Cannabis sativa L.) Extracts Against Human Antibiotic-Resistant Bacteria. Pakistan Journal of Biological Sciences, 27: 119-124.

DOI: 10.3923/pjbs.2024.119.124

URL: https://scialert.net/abstract/?doi=pjbs.2024.119.124

Pakistan Journal of Biological Sciences

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Authors

Nipaporn Armassa

Duanpen Wongsorn

Benya Saenmahayak

Somsak Rayan

Surachai Rattanasuk

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Research Article

In vitro Antibacterial Activity of Ethanolic Tanao Si Kan Dang RD1 (Cannabis sativa L.) Extracts Against Human Antibiotic-Resistant Bacteria

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