Results 11 to 20 of about 3,494,614 (161)

Sites of Biosynthesis of Outer and Inner Membrane Proteins of Neurospora crassa Mitochondria [PDF]

open access: yes, 1970
Outer and inner membranes of Neurospora crassa mitochondria were separated by the combined swelling, shrinking, sonication procedure. Membranes were characterized by electron microscopy and by marker enzyme activities.
Aasen A. J.   +14 more
core   +1 more source

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors [PDF]

open access: yes, 2020
Detergents enable the purification of membrane proteins and are indispensable reagents instructural biology. Even though a large variety of detergents have been developed in the lastcentury, the challenge remains to identify guidelines that allowfine ...
Almeida, F.   +12 more
core   +2 more sources

Intercalated Cells: More than pH Regulation

open access: yesDiseases, 2014
The renal collecting duct is the nephron segment where the final urine content of acid equivalents and inorganic ions are determined. The role of two different cell types present in this nephron segment has been determined many years ago: principal cells
Ensaf Y. Almomani   +3 more
doaj   +1 more source

Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei

open access: yesScientific Reports, 2021
Trichoderma reesei is an ascomycete fungus known for its capability to secrete high amounts of extracellular cellulose- and hemicellulose-degrading enzymes. These enzymes are utilized in the production of second-generation biofuels and T.
Sami Havukainen   +5 more
doaj   +1 more source

Rampant exchange of the structure and function of extramembrane domains between membrane and water soluble proteins. [PDF]

open access: yes, 2013
Of the membrane proteins of known structure, we found that a remarkable 67% of the water soluble domains are structurally similar to water soluble proteins of known structure. Moreover, 41% of known water soluble protein structures share a domain with an
Bowie, James U   +3 more
core   +3 more sources

X-ray crystallography reveals molecular recognition mechanism for sugar binding in a melibiose transporter MelB

open access: yesCommunications Biology, 2021
Guan and Hariharan report two crystal structures of melibiose transporter MelB in complex with substrate analogs, nitrophenyl-galactoside, and dodecyl-melibioside.
Lan Guan, Parameswaran Hariharan
doaj   +1 more source

Detection of protein concentrations using a pH-step titration method [PDF]

open access: yes, 1997
A stimulus-response method based on the application of a pH step is proposed for the detection of protein immobilized in a membrane on top of an ion-sensitive field-effect transistor (ISFET). The ISFET response to a step-wise change in pH, applied at the
Bergveld, P., Eijkel, J.C.T., Kruise, J.
core   +3 more sources

Lipid environment determines the drug-stimulated ATPase activity of P-glycoprotein

open access: yesFrontiers in Molecular Biosciences, 2023
P-glycoprotein (Pgp) is a multidrug transporter that uses the energy from ATP binding and hydrolysis to export from cells a wide variety of hydrophobic compounds including anticancer drugs, and mediates the bioavailability and pharmacokinetics of many ...
Nghi N. B. Tran   +10 more
doaj   +1 more source

A new approach to immunoFET operation [PDF]

open access: yes, 1990
A new method is presented for the detection of an immunological reaction taking place in a membrane, which covers the gate area of an ISFET. By stepwise changing the electrolyte concentration of the sample solution, a transient diffusion of ions through ...
Bergveld, P.   +3 more
core   +2 more sources

Role of PKD2 in the endoplasmic reticulum calcium homeostasis

open access: yesFrontiers in Physiology, 2022
Autosomal dominant polycystic kidney disease (ADPKD) is caused by mutations in the PKD1 or PKD2 gene which encodes membrane receptor PKD1 and cation channel PKD2, respectively. PKD2, also called transient receptor potential polycystin-2 (TRPP2), is a Ca2+
Xiong Liu, Jingfeng Tang, Xing-Zhen Chen
doaj   +1 more source

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