Results 41 to 50 of about 42,771 (161)

Characterization of 12S rRNA gene for meat identification of common wild and domestic small herbivores as an aid to wildlife forensic

open access: yesVeterinary World, 2013
Aim: Chital and sambar are the common wild small herbivores, which are vulnerable to poaching for their meat. Many times poachers claim the wild meat to be that of goat or sheep. Hence, authentic evidences are required to stop such wildlife crime.
E. Joseph   +5 more
doaj   +1 more source

Cortisol Regulates PD‐1 and IL‐12 in Canine Leishmaniasis

open access: yesParasite Immunology, Volume 48, Issue 2, February 2026.
ABSTRACT Canine visceral leishmaniasis (CanL) is a tropical zoonosis caused by Brazil's protozoan Leishmania (L.) infantum. Disorders in the hypothalamic–pituitary–adrenal (HPA) axis have been reported in human and experimental visceral leishmaniasis, but not yet in canine leishmaniasis.
Lucas Takeshi Siqueira Ito   +6 more
wiley   +1 more source

Association of the Endothelial Nitric Oxide Synthase (eNOS) G894T Gene Polymorphism With Type 2 Diabetes Mellitus in a Tunisian Population

open access: yesEndocrinology, Diabetes &Metabolism, Volume 9, Issue 1, January 2026.
The eNOS G894T polymorphism is a significant genetic risk factor for type 2 diabetes in the studied Tunisian population. The T allele appears to confer susceptibility, likely through mechanisms involving impaired eNOS function, reduced NO production and subsequent endothelial dysfunction.
Afif Ba   +5 more
wiley   +1 more source

Detection of mutations induced by CRISPR/Cas9 in cabbage protoplasts using qPCR and dPCR

open access: yesAnnals of Applied Biology, Volume 188, Issue 1, Page 260-268, January 2026.
This study evaluated two PCR‐based methods—qPCR and dPCR—for detecting CRISPR‐induced mutations in transformed cabbage protoplasts. Both methods distinguished edited from wild‐type protoplasts, offering rapid and cost‐efficient alternatives for mutation detection, with dPCR providing higher sensitivity and closer agreement with NGS results.
Ester Stajič   +2 more
wiley   +1 more source

Comparison of Microscopy and PCR-RFLP for detection of Anaplasma marginale in carrier cattle

open access: yesIranian Journal of Microbiology, 2010
Background and Objectives: In Iran, anaplasmosis is normally diagnosed with traditional Giemsa staining method. This is not applicable for identification of the carrier animals. The aim of this study was to compare the detection of Anaplasma marginale in
V Noaman, P Shayan
doaj  

Genetic Mapping of Resistance: ddRADseq‐Based QTL and Associated Polymorphism Conferring Resistance to Alpha‐Cypermethrin in Anopheles funestus

open access: yesMolecular Ecology, Volume 35, Issue 1, January 2026.
ABSTRACT The heavy reliance on pyrethroid‐based interventions has largely driven insecticide resistance in malaria vectors, accelerating the spread of resistant strains, including resistant Anopheles funestus strains. The efficacy of insecticide‐based interventions, especially insecticide‐treated nets (ITNs), the cornerstone of malaria control and ...
Talal Al‐Yazeedi   +6 more
wiley   +1 more source

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

open access: yesBrazilian Journal of Infectious Diseases
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser   +4 more
doaj   +1 more source

Effects of Lipopolysaccharide Core Modulation on Outer Membrane Protein Function and Virulence in Pectobacterium carotovorum

open access: yesMolecular Plant Pathology, Volume 27, Issue 1, January 2026.
The LPS core is critical for virulence in Pectobacterium carotovorum, as its truncation disrupts outer membrane protein assembly, reducing protease secretion, motility, and soft rot symptoms. ABSTRACT Lipopolysaccharide (LPS) is a critical component of the bacterial outer membrane, which serves as a permeability barrier and site for sensing ...
Yejin Park   +4 more
wiley   +1 more source

Molecular Characterization of Environmental Non-Tuberculous Mycobacteria Using PCR- RFLP Analysis of 441 Bp Heat Shock Protein 65 Fragments [PDF]

open access: yesIranian Journal of Public Health, 2012
Background: Non- Tuberculous Mycobacteria are environmental opportunistic pathogens that can be found in various terrestrial and aquatic habitats. There are an epidemiological links between species isolated in tap water and those isolated from patients ...
H Rezaei-Yazdi   +6 more
doaj   +2 more sources

Pepino Mosaic Virus: A Globally Important Tomato Pathogen and a Rising Model in Molecular Virology

open access: yesMolecular Plant Pathology, Volume 27, Issue 1, January 2026.
Pepino mosaic virus (PepMV) is a globally important tomato pathogen causing fruit quality losses. It comprises multiple genetic types and spreads via seed and mechanical contact. Lacking resistant cultivars, management relies on cross‐protection. PepMV–tomato interactions provide a valuable model for studying virus–plant molecular mechanisms and ...
Jesús R. Úbeda   +3 more
wiley   +1 more source

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