Results 1 to 10 of about 169,949 (334)

Insights into Transient Dimerisation of Carnitine/Acylcarnitine Carrier (SLC25A20) from Sarkosyl/PAGE, Cross-Linking Reagents, and Comparative Modelling Analysis [PDF]

open access: goldBiomolecules
The carnitine/acylcarnitine carrier (CAC) is a crucial protein for cellular energy metabolism, facilitating the exchange of acylcarnitines and free carnitine across the mitochondrial membrane, thereby enabling fatty acid β-oxidation and oxidative ...
Nicola Giangregorio   +3 more
doaj   +3 more sources

Ligand-induced movements of inner transmembrane helices of Glut1 revealed by chemical cross-linking of di-cysteine mutants. [PDF]

open access: yesPLoS ONE, 2012
The relative orientation and proximity of the pseudo-symmetrical inner transmembrane helical pairs 5/8 and 2/11 of Glut1 were analyzed by chemical cross-linking of di-cysteine mutants.
Mike Mueckler, Carol Makepeace
doaj   +9 more sources

Transmembrane helical interactions in the CFTR channel pore. [PDF]

open access: yesPLoS Computational Biology, 2017
Mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene affect CFTR protein biogenesis or its function as a chloride channel, resulting in dysregulation of epithelial fluid transport in the lung, pancreas and other organs in ...
Jhuma Das   +5 more
doaj   +3 more sources

Preparation of Polyrotaxane Fibers. Part II: Tensile Properties of Polyrotaxane Fibers Treated with Two Cross-linking Reagents [PDF]

open access: green, 2010
Polyrotaxane fibers prepared with wet spinning of polyrotaxane consisting of poly(ethylene glycol) and cyclodextrins were cross-linked with two different cross-linking reagents, i.e., divinyl sulfone (DVS) and ethylene glycol diglycidyl ether (EGDE), to ...
Abe, Koji   +6 more
core   +2 more sources

Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions [PDF]

open access: green, 2012
We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs).
Agnew, Heather D.   +11 more
core   +4 more sources

A Cofactor Regeneration System for 2‐Aminobutyric Acid Production Based on Combined Cross‐Linked Enzyme Aggregates: Utilizing His‐Tagged Enzymes With Low‐Concentration Calcium Ions as Precipitant [PDF]

open access: yesEngineering in Life Sciences
Combined cross‐linked enzyme aggregates (combi‐CLEAs) represent a promising carrier‐free immobilized enzyme technology. This study describes the preparation of combi‐CLEAs comprising leucine dehydrogenase (LeuDH) and formate dehydrogenase (FDH) for the ...
Jingran Liu   +8 more
doaj   +2 more sources

Mapping Hsp104 interactions using cross‐linking mass spectrometry [PDF]

open access: yesFEBS Open Bio
Molecular machines from the AAA+ (ATPases Associated with diverse cellular Activity) superfamily of protein disaggregases play important roles in protein folding, disaggregation and DNA processing.
Kinga Westphal   +3 more
doaj   +2 more sources

Reactive Acrylamide-Modified DNA Traps for Accurate Cross-Linking with Cysteine Residues in DNA–Protein Complexes Using Mismatch Repair Protein MutS as a Model

open access: yesMolecules, 2022
Covalent protein capture (cross-linking) by reactive DNA derivatives makes it possible to investigate structural features by fixing complexes at different stages of DNA–protein recognition.
Mayya V. Monakhova   +9 more
doaj   +1 more source

Mimicked synthetic ribosomal protein complex for benchmarking crosslinking mass spectrometry workflows

open access: yesNature Communications, 2022
Cross-linking mass spectrometry is widely used to elucidate protein structures and interactions. Here, the authors generate an extensive peptide library to benchmark the most common cross-link search engines with frequently used cross-linking reagents in
Manuel Matzinger   +5 more
doaj   +1 more source

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