Results 1 to 10 of about 214,812 (293)

Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae.

open access: yesPLoS ONE, 2023
Although many protocols have been previously developed for genomic DNA (gDNA) extraction from S. cerevisiae, to take advantage of recent advances in laboratory automation and DNA-barcode sequencing, there is a need for automated methods that can provide ...
Nina Alperovich   +2 more
doaj   +1 more source

Activity staining method of chitinase on chitin agar plate through polyacrylamide gel electrophoresis [PDF]

open access: yes, 2005
A method for detection of chitinase activity on chitin agar plate after polyacrylamide gel electrophoresis is described. Different staining dyes such as calcofluor white M2R, fluorescein isothiocyanate, rhodamine B, ruthenium red and congo red were ...
Chhatpar, HS, Gohel, Vipul, Vyas, Pranav
core   +2 more sources

A Method To Prepare An Economic Substitute For Agarose Gel Along With A Low-Cost Electrolyte For Functional DNA Gel Electrophoresis

open access: yesSyrian Journal for Science and Innovation
The use of agarose in electrophoresis is indispensable in modern biotechnology research. However, agarose is relatively expensive and not available in limited-budget scientific institutions, labs and educational facilities. Therefore, the search for more
Kamar Shayah   +4 more
doaj   +1 more source

Tomato prosystemin gene in other Solanaceae [PDF]

open access: yes, 2009
Systemin, an octadeca-peptide isolated from tomato, is a signalling molecule involved in local and systemic wound response. It regulates the activation of more than twenty defensive genes in tomato plants in response to herbivore attacks.
Araujo Burgos, Tania
core   +1 more source

Metallo-β-lactamase-production in meropenem-susceptible Pseudomonas aeruginosa isolates: risk for silent spread

open access: yesMemorias do Instituto Oswaldo Cruz, 2012
The aim of this study was to characterize two metallo-β-lactamases (MBLs)-producing Pseudomonas aeruginosa clinical isolates showing meropenem susceptibility.
Renata Cristina Picão   +6 more
doaj   +1 more source

Detection of mecA Gene in the Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

open access: yesSokoto Journal of Medical Laboratory Science, 2021
Resistance coding in Staphylococcus aureus could be phenotypic or genotypic. In genotypic coding, mecA gene codes for the production of the altered penicillin-binding protein responsible for methicillin resistance.
Raji, M.I.O.* , Haido, A.U. , Garba, I. and Shuaibu, A.B.
doaj  

Comparison between bone alkaline phosphatase immunoassay and electrophoresis technique in hemodialysis patients [PDF]

open access: yesJournal of Medical Biochemistry, 2020
Background: Problem of the variability between the different methods using for bone alkaline phosphatase (bALP) determination greately influences the clinical significance of bALP as direct marker of bone metabolism.
Milinković Neda   +3 more
doaj  

Electrophoretic separation of proteins [PDF]

open access: yes, 1981
There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins.
Ravindranath, M H, Subhashini, M H
core  

Occurrence of Extended Spectrum β-Lactamases, KPC-Type, and MCR-1.2-Producing Enterobacteriaceae from Wells, River Water, and Wastewater Treatment Plants in Oltrepò Pavese Area, Northern Italy

open access: yesFrontiers in Microbiology, 2017
To evaluate the water compartment antibiotic-resistance contamination rates, 11 wells, five streams, and four treatment plants located in the Oltrepò Pavese area were screened for the presence of third generation cephalosporins resistant Gram-negative ...
Mariasofia Caltagirone   +14 more
doaj   +1 more source

Manual for starch gel electrophoresis: A method for the detection of genetic variation [PDF]

open access: yes, 1987
The procedure to conduct horizontal starch gel electrophoresis on enzymes is described in detail. Areas covered are (I) collection and storage of specimens, (2) preparation of tissues, (3) preparation of a starch gel, (4) application of enzyme extracts ...
Aebersold, Paul B.   +4 more
core  

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