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DNA extraction and polymerase chain reaction
Journal of Cytology, 2019 DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient ...
Nalini Gupta
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Development and Evaluation of Real-Time Reverse Transcription Polymerase Chain Reaction Test for Quantitative and Qualitative Recognition of H5 Subtype of Avian Influenza Viruses [PDF]
Archives of Razi Institute, 2020 Avian influenza viruses (AIV) affect a wide range of birds and mammals, cause severe economic damage to the poultry industry, and pose a serious threat to humans.
S.G. Mirzaei, A. Shoushtari, A. Noori
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Validation of real-time polymerase chain reaction versus conventional polymerase chain reaction for diagnosis of brucellosis in cattle sera [PDF]
Veterinary World, 2021 Background and Aim: Different polymerase chain reaction (PCR) techniques have and are still being used for the direct detection of Brucella DNA in serum samples of different animal species and humans without being validated or properly validated ...
Nour H. Abdel-Hamid +5 more
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Mediterranean Journal of Infection, Microbes and Antimicrobials, 2023 Introduction: Coronavirus disease-2019 (COVID-19) has affected more than 676 million people till date, and 6,881,955 people have died from the disease as of March 28, 2023.
Müge TOYGAR DENİZ +3 more
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Optimisation of microfluidic polymerase chain reaction devices [PDF]
E3S Web of Conferences, 2021 The invention and development of Polymerase Chain Reaction (PCR) technology have revolutionised molecular biology and molecular diagnostics. There is an urgent need to optimise the performance of these devices while reducing the total construction and ...
Zagklavara Foteini +4 more
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International Journal of Mycobacteriology, 2022 Background: In this study, it was aimed to investigate Mycobacterium bovis strains isolated from lungs and lymph nodes of slaughtered animals on clonal level by using different methods such as spoligotyping, enterobacterial repetitive intergenic ...
Funda Sahin +4 more
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Identification of group A rotavirus gene 4 types by polymerase chain reaction [PDF]
Journal of Clinical Microbiology, 1992 Jon R. Gentsch +7 more
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Annals of Internal Medicine, 2020 Background: Tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on reverse transcriptase polymerase chain reaction (RT-PCR) are being used to “rule out” infection among high-risk persons, such as exposed inpatients and health ...
L. Kucirka +4 more
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Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]
Veterinary World, 2017 Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal +6 more
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