Results 191 to 200 of about 1,064,749 (319)
调控ERK1/2通路在H2S保护心肌细胞对抗阿霉素损伤中的作用
Zhongshan Daxue xuebao. Yixue kexue ban, 2013 【目的】 探讨胞外信号调节激酶1/2(ERK1/2)在阿霉素心肌毒性中的作用及硫化氢(H2S)是否通过调控ERK1/2通路抑制阿霉素的心肌毒性?【方法】 应用阿霉素处理H9c2心肌细胞建立心肌细胞毒性损伤模型;CCK-8比色法测定细胞存活率;蛋白印迹法(Western blot)检测ERK1/2蛋白的表达水平;双氯荧光素(DCFH-DA)染色荧光显微镜照相测定细胞内的活性氧(ROS)水平;罗丹明123(Rh123)染色荧光显微镜照相检测线粒体膜电位(MMP)?【结果】 5 μmol/L ...
doaj
<i>Crithidia fasciculata</i> gene of unknown function CFAC1_070014400 encodes a putative alpha/beta hydrolase with a conserved active site. [PDF]
MicroPubl BiolDavis J.
europepmc +1 more source
FEBS Letters, EarlyView.Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas +3 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 1994 本研究探讨抗原刺激粘膜免疫及封闭网状内皮系统与ＩｇＡ于肾小球系膜沉积的关系实验用８－１０周ＢＡＬＢ／ｃ雌性小鼠６０只，分别口服饮食抗原（麦胶蛋白和牛－γ球蛋白），并于实验开始后前３周用或不用印度墨水尾静脉注射。实验第１４周后杀检，作肾组织病理检查。结果表明：单纯口服饮食抗原组，免疫荧光显示，肾小球系膜ＩｇＡ阳性率为（５８％～６７％）；口服抗原加网状内皮系统封闭组，则为（７３％～８３％），ＩｇＡ荧光稍强于ＩｇＧ。光镜和电镜结果均提示肾小球系膜区增生；网状内皮系统功能在１４周明显减弱。２４ｈ尿蛋白无异常 ...
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Re-evaluating the role of <i>hok</i> /Sok in T4 phage defense. [PDF]
MicroPubl BiolMessineo A +3 more
europepmc +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2014 【目的】 探究小鼠胚胎干细胞(ESC)条件培养基(CM)对人类脂肪干细胞(ADSC)增殖速率的促进作用,并研究CM对ADSC表面标志表达以及成骨成脂分化的影响?【方法】 胶原酶I消化法提取人ADSC?培养2代后分普通组?40%CM组和40%ES组,分别以普通ADSC培养液?普通ADSC培养液加40%CM和普通ADSC培养液加40%ES培养液处理?3代后,用MTT法检测细胞增殖活性,流式细胞仪检测实验前后细胞表面标志的表达;诱导其向成骨成脂分化并鉴定?【结果】MTT法检测,40 ...
doaj
The <i>grooveless</i> gene encodes a single von Willebrand factor type C domain protein necessary for proper formation of the scutoscutellar sulcus in <i>Drosophila melanogaster</i>. [PDF]
MicroPubl BiolCook KR, Mauthner SE.
europepmc +1 more source
The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes
FEBS Letters, EarlyView.Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga +3 more
wiley +1 more source
Mycobacteria isolated from temperate stony corals. [PDF]
MicroPubl BiolUlanova D, Mezaki T, Kubota S.
europepmc +1 more source