The Effect of Vitamin D3 Supplementation on the Incidence of Diagnosed Dementia Among Healthy Older Adults-The Finnish Vitamin D Trial. [PDF]
Lönnroos E+9 more
europepmc +1 more source
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada+3 more
wiley +1 more source
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo+4 more
wiley +1 more source
Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing. [PDF]
Cong R+10 more
europepmc +1 more source
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Urinary Metabolic Biomarkers of Attentional Control in Children With Attention-Deficit/Hyperactivity Disorder: A Dimensional Approach Through <sup>1</sup>H NMR-Based Metabolomics. [PDF]
Salmerón ADM+6 more
europepmc +1 more source
Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa+2 more
wiley +1 more source
Associations of water intake and Intra-Meal fluid consumption with obesity, insulin resistance, and predictors of cardiovascular diseases among Iranian women. [PDF]
Moosavi H+4 more
europepmc +1 more source
Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni+4 more
wiley +1 more source