Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Design, synthesis, in-vivo, and in-silico studies of 1,2,3-triazole tethered derivatives of morphine as novel anti-nociceptive agents. [PDF]
Nourmandipour F+6 more
europepmc +1 more source
Consumer perspectives on implementing falls prevention and management in rehabilitation hospitals: protocol for a qualitative study. [PDF]
McKercher JP+6 more
europepmc +1 more source
Effectiveness of Negative Pressure Wound Therapy With Instillation and Dwell in Removing Nonviable Tissue, Promoting Granulation Tissue, and Reducing Surgical Debridements: A Systematic Literature Review. [PDF]
Acosta J+5 more
europepmc +1 more source
Associations of water intake and Intra-Meal fluid consumption with obesity, insulin resistance, and predictors of cardiovascular diseases among Iranian women. [PDF]
Moosavi H+4 more
europepmc +1 more source
Vitamin D status and its determinants among Chinese infants and toddlers in Hong Kong. [PDF]
Tung KTS+11 more
europepmc +1 more source
Suppression of gut colonization by multidrug-resistant Escherichia coli clinical isolates through cooperative niche exclusion. [PDF]
Wende M+15 more
europepmc +1 more source