Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
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Multi-omics analysis reveals Parabacteroides distasonis and Kineothrix alysoides as potential key bacterial species associated with anemia in sows. [PDF]
Sun L +6 more
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Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
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Investigating the Impact of Carboxylated Polystyrene Nanoplastics in the Liver Using Cell Lines and Precision-Cut Liver Slices. [PDF]
Pandey N +9 more
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Active ROP2 triggers leaf senescence and orchestrates the growth-senescence trade-off under nitrogen starvation. [PDF]
Zhang T +8 more
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RETRACTION: S. Gopinath, K. Alapati, R. R. Malla, C. S. Gondi, S. Mohanam, D. H. Dinh, and J. S. Rao, “Mechanism of p27 Upregulation Induced by Downregulation of Cathepsin B and Upar in Glioma,” Molecular Oncology 5, no. 5 (2011): 426–437, https://doi.org/10.1016/j.molonc.2011.07.004.
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Synthesis, Characterization, Antioxidant Evaluation, Cytotoxicity Studies, and Molecular Docking of Novel Sulfonamide Derivatives. [PDF]
Bouraoui O +8 more
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Association of Serum Vitamin D Levels and Ventricular Dysfunction Among Patients With Acute ST-Segment Elevation Myocardial Infarction in a Tertiary Care Hospital. [PDF]
Arora K +3 more
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Distinct D-box motifs in SPD-2 mediate APC/CFZR-1-dependent degradation and centrosomal localization in Caenorhabditis elegans embryos. [PDF]
Yim RN, DiPanni JR, Rivera PA, Song MH.
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