Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
The Results of the "My Challenge" Camp in Addressing Childhood Obesity. [PDF]
Poklar Vatovec T +4 more
europepmc +1 more source
Prognostic Role of HER2 Status and Adjuvant Trastuzumab Treatment in Lymph Node-Negative Breast Cancer Patients - a Retrospective Single Center Analysis. [PDF]
Fokter Dovnik N +5 more
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero +5 more
wiley +1 more source
Patient Reported Indicator Surveys (Paris): Methodological Considerations of a Field Trial in Slovenia. [PDF]
Ambrož M +3 more
europepmc +1 more source
Computed Tomographic Perfusion Imaging for the Prediction of Response and Survival to Transarterial Chemoembolization of Hepatocellular Carcinoma. [PDF]
Popovic P +5 more
europepmc +1 more source
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
Barney Warf in Santa Arias (Ur.): The Spatial Turn: Interdisciplinary Perspectives
Marjeta Pisk
doaj
Genetic differentiation of the Western Capercaillie highlights the importance of South-eastern Europe for understanding the species phylogeography. [PDF]
Bajc M +9 more
europepmc +1 more source

