Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source
Prognostic Role of HER2 Status and Adjuvant Trastuzumab Treatment in Lymph Node-Negative Breast Cancer Patients - a Retrospective Single Center Analysis. [PDF]
Fokter Dovnik N +5 more
europepmc +1 more source
In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans
In vitro induction of human regulatory T cells using standard protocols generates suppressive, Foxp3‐expressing CD4+ T cells independently of TGF‐β supplementation and donor age. Regulatory T (Treg) cells play a key role in immune tolerance and homeostasis.
Lynn Muller +3 more
wiley +1 more source
Understanding the Second Year of the COVID-19 Pandemic From a Nursing Perspective: A Multi-Country Descriptive Study. [PDF]
Squires A +26 more
europepmc +1 more source
Intralingual Variation in Acceptability Judgments and Production: Three Case Studies in Russian Grammar. [PDF]
Gerasimova A, Lyutikova E.
europepmc +1 more source
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li +7 more
wiley +1 more source
Patient Reported Indicator Surveys (Paris): Methodological Considerations of a Field Trial in Slovenia. [PDF]
Ambrož M +3 more
europepmc +1 more source
Genetic differentiation of the Western Capercaillie highlights the importance of South-eastern Europe for understanding the species phylogeography. [PDF]
Bajc M +9 more
europepmc +1 more source
Prediction of The Trifecta And Pentafecta İn Minimal İnvasive Partial Nephrectomy
Deniz Bolat, Bulent Gunlusoy
openaire +1 more source
The PAM pathway and its impact on cellular metabolism. Color‐coded key symbols represent Rapamycin (R, orange, targeting mTOR) and Dactolisib (D, green, targeting PI3K/mTOR), indicating their sites of action. Created with BioRenders.com. Glioblastoma resists therapy through interconnected survival programs.
Kai Zhao +23 more
wiley +1 more source

