Results 31 to 40 of about 1,000 (178)
Overexpression of NbNBR1 promotes PVX-βC1 mediated symptom and enhances the protein level of βC1.
(A, C) Symptoms of the PVX- or PVX-βC1-inoculated NbNBR1-overexpressed transgenic N. benthamiana plants at 5 dpi. Bar = 2 cm. (B, D) Western blot analyses of PVX CP and βC1 accumulations in the assayed plants.
Xueping Zhou (131154) +4 more
core +1 more source
NbNBR1 prevents βC1 degradation by perturbing the interaction of NbRFP1 and βC1.
(A) Y3H assay was used to determine the interaction among βC1, NbRFP1, and NbNBR1. Yeast cell culture co-expressing AD-βC1+Bridge-NbRFP1-MBP was used as a control. Different yeast cell cultures transformed with the indicated plasmids were grown on the SD/
Xueping Zhou (131154) +4 more
core +1 more source
NbNBR1 could affect the βC1-mediated disease symptom formation and the accumulation of βC1.
(A, C) Symptoms of the PVX- or PVX-βC1-inoculated Wt, NbNBR1-knockout N. benthamiana plants at 5 dpi. Bar = 2 cm. (B, D) Western blot analyses of PVX CP and βC1 accumulations in the assayed plants.
Xueping Zhou (131154) +4 more
core +1 more source
NbNBR1 expression is up-regulated by βC1.
(A) Growth phenotypes of the Wt and the HA-βC1 transgenic N. benthamiana plants. (B) Expression of NbNBR1 in the Wt and the HA-βC1 transgenic plants was confirmed through qRT-PCR (*pt test).
Xueping Zhou (131154) +4 more
core +1 more source
Viral βC1 protein disrupts the ATG3-GAPC interaction and thereby induces autophagy. Autophagy plays an important role in plant–pathogen interactions. Several pathogens including viruses induce autophagy in plants, but the underpinning mechanism remains ...
Asigul Ismayil +12 more
semanticscholar +1 more source
DNA β is a single-stranded satellite DNA which encodes a single gene, βC1. To better understand the role of βC1 in the pathogenicity of DNA β, a yeast two-hybrid screen of a tomato cDNA library was carried out using βC1 from Cotton leaf curl Multan virus
Omid Eini +5 more
doaj +1 more source
Suppression of RNA silencing by a plant DNA virus satellite requires a host calmodulin-like protein to repress RDR6 expression. [PDF]
In plants, RNA silencing plays a key role in antiviral defense. To counteract host defense, plant viruses encode viral suppressors of RNA silencing (VSRs) that target different effector molecules in the RNA silencing pathway.
Fangfang Li +3 more
doaj +1 more source
Co-expression of Myc-NbNBR1 increases βC1 accumulation.
(A) Myc-NbNBR1, YFP, and βC1-YFP were transiently co-expressed, as indicated, in N. benthamiana leaves followed by Western blot assays using anti-GFP or anti-Myc antibodies.
Xueping Zhou (131154) +4 more
core +1 more source
Tomato yellow leaf curl China virus (TYLCCNV) is a monopartite begomovirus associated with different betasatellites. In this study, we investigate two different isolates of Tomato yellow leaf curl China betasatellite (TYLCCNB) to determine what features ...
Jie Zhang +3 more
doaj +1 more source
A single amino acid substitution in βC1 (βC1K4A) abolishes the interaction between βC1 and NbNBR1.
(A) Yeast cell cultures transformed with the indicated plasmids were 10-fold diluted and then grown on the SD/-Trp-Leu or SD/-Trp-Leu-His-Ade medium. Yeast cell culture co-transformed with AD-βC1+BD-NbNBR1 was used as the positive control.
Xueping Zhou (131154) +4 more
core +1 more source

