鞘氨醇-1-磷酸通过“成血管-成骨”偶联促进强直性脊柱炎患者异常骨化
目的探究鞘氨醇-1-磷酸(S1P)在强直性脊柱炎(AS)异常骨化中的作用,阐明S1P与“成血管-成骨”偶联的关系,为AS治疗提供新的方法和策略。方法收集AS患者和普通髋关节置换患者的股骨头,进行免疫组织化学染色评估成骨水平和H型血管形成情况;体外通过ELISA检测AS患者来源的间充质干细胞(ASMSCs)或健康供体来源的间充质干细胞(HDMSCs)的成骨分化过程中S1P的合成情况及S1P通路相关分子表达情况,评估S1P通路激活情况,分别干扰MSC中鞘氨醇激酶1(SK1)表达和添加S1PR调节剂芬戈莫德 ...
米汝佳 +5 more
doaj
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