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慢病毒载体介导IL-24基因转导人骨髓间充质干细胞的实验研究

Zhongguo fei ai za zhi = Chinese journal of lung cancer, 2013
背景与目的 以人骨髓间充质干细胞(human bone marrow mesenchymal stem cells, hBMSCs)作为抑癌基因IL-24的细胞载体的研究目前未见报道。应用Gateway法构建共表达增强型绿色荧光蛋白(enhanced green fluorescent protein, EGFP)基因和IL-24基因的慢病毒载体,探讨其对hBMSCs的转导情况,为今后肿瘤的基因治疗奠定基础。 方法 应用DNA重组技术构建含有IL-24和EGFP基因的慢病毒表达载体,并与慢病毒包装系统 ...
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