[Prokaryotic expression of a recombinant protein of adeno-associated virus capsid conserved regions and preparation of its polyclonal antibody]. [PDF]
Nan Fang Yi Ke Da Xue Xue Bao, 2022 Li S +8 more
europepmc +1 more source
Zhongguo shiyan zhenduanxue, 2018 目的探究自合成铕螯合剂对甲胎蛋白单克隆抗体的标记方法。方法经EDC和NHS活化的铕螯合剂偶联甲胎蛋白单克隆抗体,通过透析收集标记抗体,检测荧光强度和免疫活性,计算回收率和标记率。结果标记抗体荧光强度高,免疫活性无明显变化,回收率为94%,标记率为15。结论自合成的铕螯合剂可用于甲胎蛋白单克隆抗体的标记,标记效果理想,可进一步建立均相时间分辨荧光免疫分析方法。
孙延文 +5 more
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】构建以基因佐剂hGM-CSF基因为基础的真核表达质粒pIGM-CSF,并在宫颈癌细胞HeLa中表达与鉴定。【方法】采用聚合酶链反应从载体pORF-hGM-CSF中,扩增出hGM-CSF基因,克隆到双顺反子真核表达载体pIRES的多克隆位点B（MCSB）中,构建pIGM-CSF真核表达载体。然后采用脂质体转染法将其转染HeLa细胞,用RT-PCR在RNA水平和ELISA在蛋白水平检测其在真核细胞中的表达。【结果】所克隆hGM-CSF基因片段经测序完全正确;重组质粒转染Hela细胞后,检测有hGM ...
卢燕茹, 江振友
doaj
Zhongguo shiyan zhenduanxue, 2007 目的构建人白细胞介素-15（interleukin 15,IL-15）原核表达克隆并在大肠杆菌中表达。方法从经脂多糖刺激的外周血单核细胞中提出mRNA,RT-PCR扩增出IL-15成熟肽前体蛋白基因,构建原核表达克隆（PGEX-4T-2/IL-15）。IPTG诱导表达目的蛋白,SDS-PAGE电泳分析蛋白图谱。Western blot鉴定表达蛋白。结果经酶切和测序分析,表明成功构建了pGEX-4T-2/IL-15原核表达克隆;经IPTG诱导,转化的大肠杆菌表达出相对分子质量（Mr）为42 ...
董学东, 朱元祺, 姜岩
doaj
[Indolent γδT-cell clone in Felty syndrome: a case report and literature review]. [PDF]
Zhonghua Xue Ye Xue Za ZhiWang C, Fu MW, An G, Huang WY, Yang LJ.
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】 FAM3A (family with sequence similarity 3, member A)为新近发现的细胞因子FAM3的家族成员之一,本研究检测FAM3A的相互作用蛋白? 【方法】 克隆FAM3A并连接到pGB载体,转化酵母Y190宿主菌,加入含主要信号传导细胞因子人cDNA文库,按标准程序进行酵母双杂交,以X-Gal显色评估活性?提取扩增阳性克隆,连接pACT2载体,测序?检索基因库确定阳性克隆基因?克隆阳性基因并与FAM3A质粒共转染酵母进一步确定相互作用?
doaj
[The state of the art: diagnosis and therapeutic interventions of clonal hematopoiesis of indeterminate potential and clonal cytopenias of undetermined significance]. [PDF]
Zhonghua Xue Ye Xue Za ZhiXiao ZJ.
europepmc +1 more source
[IgM- κ and IgM- λ biclonal POEMS syndrome combined with essential thrombocythemia: a case report]. [PDF]
Zhonghua Xue Ye Xue Za ZhiWang J, Wu F, Zhai ZM.
europepmc +1 more source
[High-throughput sequencing in identifying somatic hypermutation in immunoglobulin heavy chain variable regions with complex clonal backgrounds]. [PDF]
Zhonghua Xue Ye Xue Za ZhiGao MG +5 more
europepmc +1 more source
[Characteristics of immunoglobulin heavy-chain gene clonal rearrangements by next-generation sequencing of patients with multiple myeloma]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2021 Yao L +7 more
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