Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】 FAM3A (family with sequence similarity 3, member A)为新近发现的细胞因子FAM3的家族成员之一,本研究检测FAM3A的相互作用蛋白? 【方法】 克隆FAM3A并连接到pGB载体,转化酵母Y190宿主菌,加入含主要信号传导细胞因子人cDNA文库,按标准程序进行酵母双杂交,以X-Gal显色评估活性?提取扩增阳性克隆,连接pACT2载体,测序?检索基因库确定阳性克隆基因?克隆阳性基因并与FAM3A质粒共转染酵母进一步确定相互作用?
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Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】构建以基因佐剂hGM-CSF基因为基础的真核表达质粒pIGM-CSF,并在宫颈癌细胞HeLa中表达与鉴定。【方法】采用聚合酶链反应从载体pORF-hGM-CSF中,扩增出hGM-CSF基因,克隆到双顺反子真核表达载体pIRES的多克隆位点B（MCSB）中,构建pIGM-CSF真核表达载体。然后采用脂质体转染法将其转染HeLa细胞,用RT-PCR在RNA水平和ELISA在蛋白水平检测其在真核细胞中的表达。【结果】所克隆hGM-CSF基因片段经测序完全正确;重组质粒转染Hela细胞后,检测有hGM ...
卢燕茹, 江振友
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[Characteristics of immunoglobulin heavy-chain gene clonal rearrangements by next-generation sequencing of patients with multiple myeloma]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2021 Yao L +7 more
europepmc +1 more source
Zhongguo shiyan zhenduanxue, 2007 目的构建人白细胞介素-15（interleukin 15,IL-15）原核表达克隆并在大肠杆菌中表达。方法从经脂多糖刺激的外周血单核细胞中提出mRNA,RT-PCR扩增出IL-15成熟肽前体蛋白基因,构建原核表达克隆（PGEX-4T-2/IL-15）。IPTG诱导表达目的蛋白,SDS-PAGE电泳分析蛋白图谱。Western blot鉴定表达蛋白。结果经酶切和测序分析,表明成功构建了pGEX-4T-2/IL-15原核表达克隆;经IPTG诱导,转化的大肠杆菌表达出相对分子质量（Mr）为42 ...
董学东, 朱元祺, 姜岩
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Zhongguo shiyan zhenduanxue, 2007 目的获得与人前列腺癌细胞系PC3M细胞特异结合的短肽,作为人前列腺癌靶向治疗的先导化合物。方法以PC3M细胞为靶细胞,正常人前列腺细胞为吸附细胞对噬菌体7肽库进行差减筛选,用细胞ELISA、免疫荧光细胞化学染色鉴定阳性噬菌体克隆并测序。结果经三轮筛选,从随机挑选的10个噬菌体克隆中得到8个能特异性与PC3M细胞结合,而不与正常人前列腺细胞结合的阳性克隆。其氨基酸序列有一定同源性。结论得到多个序列不同的特异性结合PC3M细胞的噬菌体克隆。本实验获得的短肽具有一定的肿瘤亲合力和特异性 ...
夏阳 +4 more
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[The state of the art: diagnosis and therapeutic interventions of clonal hematopoiesis of indeterminate potential and clonal cytopenias of undetermined significance]. [PDF]
Zhonghua Xue Ye Xue Za ZhiXiao ZJ.
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[IgM- κ and IgM- λ biclonal POEMS syndrome combined with essential thrombocythemia: a case report]. [PDF]
Zhonghua Xue Ye Xue Za ZhiWang J, Wu F, Zhai ZM.
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[Lymphoplasmacytic lymphoma patient with biclonal IgG and IgA: a case report]. [PDF]
Zhonghua Xue Ye Xue Za Zhi, 2020 Zhi YJ, Zeng WQ, Li Q.
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[High-throughput sequencing in identifying somatic hypermutation in immunoglobulin heavy chain variable regions with complex clonal backgrounds]. [PDF]
Zhonghua Xue Ye Xue Za ZhiGao MG +5 more
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[Chinese expert consensus on screening and clinical application of monoclonal immunoglobulins(2025)]. [PDF]
Zhonghua Xue Ye Xue Za ZhiHematology Society of China Medicine Education Association +5 more
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