Results 31 to 40 of about 279,159 (155)

动物克隆与克隆技术

open access: yes野生动物学报, 2003
自从克隆羊“多莉”诞生之后,克隆一词便成为了传媒追逐的热点,那么究竟什么是克隆呢?克隆一词源于英文“clone”的中文译音,意为无性繁殖,即通过非有性的过程复制个体,不通过性细胞结合而繁殖的过程。植物的克隆现象很常见,植物块茎的自然繁殖、扦插、压条和嫁接都属于无性繁殖,均属于植物的克隆行为。动物克隆则不同 ...
孙忻, 王丽
doaj  

General‐purpose genotypes and evolution of higher plasticity in clonality underlie knotweed invasion

open access: yesNew Phytologist, Volume 246, Issue 2, Page 758-768, April 2025.
Summary Many widespread invasive plant species express high phenotypic variation across novel environments, providing a unique opportunity to examine ecological and evolutionary dynamics under global change. However, studies often lack information about the origin of introduced populations, limiting our understanding of post‐introduction evolution.
Shengyu Wang   +15 more
wiley   +1 more source

克隆浅释

open access: yesZhongguo shiyan zhenduanxue, 1997
克隆浅释于永利作者单位:130021长春,白求恩医科大学免疫学教研室关于克隆羊的报道在全世界引起了轩然大波,一时间“克隆”成了一个十分风行的词。本文将从医学的角度对克隆做一简要的解释。克隆(clone)一词来源于希腊文(Klon),指从一个个体经无性...
于永利
doaj  

Long‐term use of lokivetmab in dogs with atopic dermatitis

open access: yesVeterinary Dermatology, Volume 35, Issue 6, Page 683-693, December 2024.
Background – Lokivetmab, a caninised monoclonal antibody against interleukin (IL)‐31, is an effective treatment for the pruritus associated with canine atopic dermatitis (cAD). Objectives – To investigate the efficacy and safety of lokivetmab during long‐term treatment defined as at least three consecutive lokivetmab injections in atopic dogs under ...
Bettina Kasper   +2 more
wiley   +1 more source

A multicentric intermediate‐size B‐cell lymphoma with epitheliotropism in a Freiberger mare

open access: yesVeterinary Dermatology, Volume 35, Issue 5, Page 573-577, October 2024.
This report describes a multicentric intermediate‐size B‐cell lymphoma with epitheliotropism in a Freiberger mare affecting multiple mucous membranes, skin and internal organs. The clonal neoplastic B‐cell population was accompanied by numerous reactive polyclonal small T cells. Differential diagnoses for these unusual findings are discussed.
Inês Berenguer Veiga   +6 more
wiley   +1 more source

Prospective diagnostic test accuracy of transvaginal ultrasound posterior approach for uterosacral ligament and torus uterinus deep endometriosis

open access: yesUltrasound in Obstetrics &Gynecology, Volume 63, Issue 2, Page 263-270, February 2024.
ABSTRACT Objective To determine the diagnostic test accuracy of transvaginal ultrasound (TVS) using a standardized technique for the diagnosis of deep endometriosis (DE) of the uterosacral ligaments (USLs) and adjacent torus uterinus (TU). Methods This was a prospective diagnostic test accuracy study conducted at the McMaster University Medical Center ...
S. M. Freger   +3 more
wiley   +1 more source

新疆额尔齐斯河水系银鲫克隆多样性研究 [PDF]

open access: yes, 2009
银鲫(Carassius auratus gibelio Bloch)由于其独特的遗传背景和繁殖方式而成为研究进化遗传学和选择育种的一个独特的模式生物。到目前为止,我们对新疆额尔齐斯河水系银鲫群体的多样性状况一直知之甚少。为了更好地了解额尔齐斯河水系银鲫群体的克隆多样性状况,本研究中,我们采集了来自新疆额尔齐斯河水系的4个鲫鱼群体。通过流式细胞术分析血细胞样品,结果证实这些鲫鱼均为三倍体银鲫。通过血清转铁蛋白电泳表型分析,我们从这些银鲫群体中鉴定出总共8个不同的克隆。在这些鉴定的克隆中,有4个克隆 ...
李风波, 桂建芳, 周莉
core  

一种简便有效的PCR扩增片段直接回收克隆方法

open access: yes四川大学学报. 自然科学版, 2007
作者提供一种简便快速的克隆方法,即直接对琼脂糖凝胶电泳分离的PCR产物进行克隆,可获得较高的阳性克隆率.结果显示,300~700 bp大小的片段阳性克隆率为70.72%,300 bp以下和大于700 bp的片段阳性克隆率分别达到60.38%和45.33%.该方法适用于含有大量不同大小DNA片段的PCR产物并需要同时对其进行回收和克隆的样品,不仅可以大大简化实验过程,也可以降低假阳性出现的机会.
康耀霞, 张义正
doaj  

再生障碍性贫血儿童TCR Vβ亚家族T细胞克隆性增殖及其与HLA—DRB1*15的关系

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2006
【目的】探讨再生障碍性贫血(再障)儿童TCR Vβ24个亚家族T细胞克隆性及其与HLA—DRB1*15的关系。【方法】再障儿童17例(SAA14例,MAA3例),采用RT—PCR和基因扫描分析外周血或骨髓TCR Vβ24个亚家族基因的表达和克隆性,SSP—PCR检测HLA—DR。【结果】再障儿童外周血T细胞仅表达4—22个Vβ亚家族,而正常儿童外周血T细胞几乎表达所有Vβ亚家族。12例(包括初诊SAA4例,CR4例,复发1例和MAA3例)再障儿童存在不同程度T细胞克隆性增殖,但个体间差异较大 ...
黄永兰   +4 more
doaj  

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