[Molecular imprinting strategies and advances targeting biomembranes]. [PDF]
Se PuYuan XT, Wang L, Chen LX, Hu LH.
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Zhongguo shiyan zhenduanxue肝细胞癌（HCC）是世界上第六大常见癌症[1]，也是导致癌症相关死亡的第三大常见原因，一些生物标志物已被报道为肝癌的肿瘤标志物，例如α-胎蛋白（AFP）、甲胎蛋白异质体（AFP-L3）和des-γ-羧基凝血酶原（DCP）[2-3]。本实验通过免疫印迹、免疫组化方法分析AFP-L3在肝癌的表达水平，从而为肝癌的诊断提供辅助检查。
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[Design and synthesis of molecularly imprinted polymers and their applications in medical diagnosis and biocatalysis]. [PDF]
Se PuSong Q, Li X, Lyu Y.
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[Research progress of molecular imprinting technology in the field of disease diagnosis and therapy]. [PDF]
Se PuZhu R, Cai GP, Zheng HJ, Jia Q.
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[Advances in application of cell imprinting technology in biomedical field]. [PDF]
Se PuZhao XM, Zhang ZY, Sun WJ, Qing GY.
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[Application of molecularly imprinted polymers-based sensors for determination of acute coronary syndrome biomarkers]. [PDF]
Se PuZhang Y +6 more
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[Expression and significance of the <i>FABP6</i> long transcript in clear cell renal cell carcinoma]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanYin H +5 more
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慢性吗啡诱导HEK293细胞GIRK1和GIRK2膜表达下调
Zhongshan Daxue xuebao. Yixue kexue ban目的利用人胚胎肾细胞293（HEK293）观察G蛋白门控内向整流钾离子通道（GIRK）亚单位GIRK1和GIRK2在吗啡耐受后的表达和分布变化。方法使用HEK293细胞转染慢病毒pLV-CMV-GIRK1-T2A-GIRK2-P2A-MOR，构建过表达GIRK1、GIRK2和μ阿片受体（MOR）的稳转HEK293细胞，使用吗啡（1 μmol/L，24 h）处理该稳转细胞，构建吗啡耐受HEK293细胞模型。细胞免疫荧光检测细胞模型中MOR与GIRK1和GIRK2的分布及共定位情况 ...
刘冰柯, 崔宇, 韩雪, 肖力
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[Human umbilical cord mesenchymal stem cells protect against neonatal white matter injury by activating the Nrf2/Keap1/HO-1 signaling pathway]. [PDF]
Zhongguo Dang Dai Er Ke Za ZhiWang C, Wang MX, Zhu YP.
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华支睾吸虫的一个 μ 型 GST 基因的表达、纯化与免疫反应性鉴定
Zhongshan Daxue xuebao. Yixue kexue ban, 2004 【目的】扩增华支睾吸虫一个μ型谷胱甘肽移酶(mu-classglutathionetransferaseofClonorchissinen-sis,CsGSTM)基因,明确是否存在内含子,进行原核表达,纯化其表达产物,鉴定免疫反应性。【方法】用PCR方法从成虫cDNA文库和基因组中扩增出目的基因,测序,定向克隆到原核表达载体pET-30a(+),在大肠杆菌BL21/DE3表达,按照Ni-NTAagarose说明书纯化重组蛋白,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodiumdode ...
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