Results 21 to 30 of about 6,226 (160)

Detection and Inhibition of IgE for cross‐reactive carbohydrate determinants evident in an enzyme‐linked immunosorbent assay for detection of allergen‐specific IgE in the sera of dogs and cats

open access: yesVeterinary Dermatology, Volume 31, Issue 6, Page 439-e116, December 2020., 2020
Background It has been demonstrated recently that immunoglobulin (Ig)E specific for cross‐reactive carbohydrate determinants (CCD) is present in the serum of allergen‐sensitized dogs and cats, and that these CCD‐specific antibodies might confound serological testing. Hypothesis/Objective The objective was to document the prevalence of CCD detectable in
Kenneth W. Lee   +3 more
wiley   +1 more source

Discrepancies in the bilateral intradermal test and serum tests in atopic horses

open access: yesVeterinary Dermatology, Volume 31, Issue 5, Page 390-e104, October 2020., 2020
Background – In equine atopic patients intradermal testing (IDT) and immunoglobulin (Ig)E serology are used frequently. There is little evidence regarding the reproducibility of the IDT and IgE serology in horses. Objectives – To compare the results of a simultaneously performed IDT on the left and right side of the neck in atopic horses, and to ...
Catharina M. M. van Damme   +2 more
wiley   +1 more source

乌鳢免疫球蛋白M重链和免疫球蛋白轻链的克隆与特征分析 [PDF]

open access: yes, 2010
应用RACE-PCR方法克隆并鉴定了乌鳢免疫球蛋白M(i mmunoglobulin M,Ig M)重链(H)和免疫球蛋白轻链(L)全长cDNA。乌鳢IgH cDNA全长1 912个核苷酸(nt),包含1 797 nt的开放阅读框,编码598个氨基酸(aa)。IgH恒定区(CH)均包含1对保守的半胱氨酸残基形成链内二硫键,其中CH1区氨基末端存在保守的半胱氨酸残基与轻链形成链间二硫键,4个推测的N-糖基化位点(NXT和NXS)则分别位于CH1、CH2和CH4 ...
周秀霞, 贾伟章
core  

Long‐lasting otic solution containing mometasone furoate can influence intradermal testing in dogs with healthy ears and otitis externa

open access: yesVeterinary Dermatology, Volume 35, Issue 6, Page 736-744, December 2024.
Background – Topical therapy is preferred for otitis externa (OE) in dogs, and otic products commonly contain glucocorticoids that can be systemically absorbed and possibly interfere with diagnostic tests such as intradermal testing (IDT). Hypothesis/Objectives – To determine the effect of a long‐lasting otic solution containing mometasone furoate (MF)
Jennifer L. Clegg   +2 more
wiley   +1 more source

Roles of Netrin-1/Slit3 in the metastasis of fibrosarcoma [PDF]

open access: yes, 2016
神经导向因子Netrins及Slits是人们在研究神经系统发育过程中被发现的,在神经轴突穿越中线的过程中,Slit/Robo信号与Netrin/DCC信号之间的协调互作对轴突顺利穿越中线并到达对侧靶点至关重要。在近年的研究中发现,Netrins及Slits家族对肿瘤的生长和转移也具有重要的作用,然而关于Netrin-1与Slit3及其相互作用在纤维肉瘤中的作用还鲜有报道。成纤维肉瘤细胞HT1080是高转移性的恶性成纤维肉瘤细胞,是研究肿瘤侵袭转移的常用模型,本论文对Netrin ...
郑龙庆
core  

Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor [PDF]

open access: yes, 2017
[目的]建立稳定表达融合EGFP的人新生儿Fc受体(h FcRn)的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5 ...
周兵   +6 more
core   +1 more source

Establishment of An Effective System to Develop Avian Polyclonal Antibodies and Its Primary Application [PDF]

open access: yes, 2016
小鼠单克隆抗体技术已成为生物医药领域不可或缺的研究工具,在疾病的诊断和治疗领域也得到广泛应用。然而,由于小鼠自身免疫系统的局限,某些抗原或表位,特别是同为哺乳动物的人体抗原在小鼠体内可能因为种属相近而不易产生好的免疫应答,给制备相应的小鼠单克隆抗体造成一定的困难。因此,有必要基于其它种属动物开发新的抗体制备技术。禽源抗体技术也称卵黄抗体技术(简称IgY技术),是用免疫原刺激禽类动物,产生相应的特异性抗体,该抗体会转运、储存在卵黄中,经特殊的提取技术,可从卵黄中获得大量特异性的多克隆抗体的一种抗体制备技术。
康雅虹
core  

血清免疫固定电泳、蛋白电泳、免疫球蛋白及轻链定量对多发性骨髓瘤临床诊断价值探讨

open access: yesZhongguo shiyan zhenduanxue, 2010
目的探讨血清蛋白电泳、总蛋白、免疫球蛋白及轻链、蛋白电泳、免疫固定电泳测定对多发性骨髓瘤(MM)的诊断价值。方法对89例多发性骨髓瘤患者的血清总蛋白、免疫球蛋白及轻链、蛋白电泳、免疫固定电泳和尿轻链κ、λ的检测结果进行统计分析。结果 89例MM患者38例患者血清总蛋白含量升高;55例免疫球蛋白及轻链定量显著升高;血清蛋白电泳56例检出M带,而免疫固定电泳中89例均为阳性结果。结论免疫固定电泳检是MM诊断的敏感方法 ,应作为MM的常规检测方法 。
刘汐盈, 孙淑艳, 宋媛媛
doaj  

The APSIM manure module: improvements in predictability and application to laboratory studies [PDF]

open access: yes, 2012
应用RACE方法获得斜带石斑鱼膜结合型免疫球蛋白M(membrane-bound immu-noglobulin M,mIgM),膜结合型免疫球蛋白D(mIgD),分泌型免疫球蛋白Z(secretory immu-noglobulin Z,sIgZ)的重链基因。斜带石斑鱼膜结合型IgM重链恒定区包含3个恒定区结构域(μ1,μ2,μ3)以及两个跨膜外显子(TM1,TM2),TM1外显子与μ3结构域末端相连接。氨基酸序列相似性分析结果显示,斜带石斑鱼mIgM各恒定区与牙鲆mIgM恒定区相似性最高,为53 ...
Delve, Robert J.   +3 more
core  

轻链多聚体形成导致结果异常的1例轻链型骨髓瘤病例报道

open access: yesZhongguo shiyan zhenduanxue, 2020
骨髓瘤是由浆细胞发展而成的恶性肿瘤,以骨髓浆细胞异常增生及过度生成单克隆免疫球蛋白或游离κ或γ轻链为显著特征。轻链型是其中的一种类型,以单一分泌免疫球蛋白的一种轻链为特征,并且容易形成轻链多聚体的形式,对尿蛋白电泳检测尿蛋白和散射速率比浊法定量检测血尿轻链产生影响[1]。
柏玉, 徐志晔, 王丽, 夏茂
doaj  

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