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深低温反复冻融杀伤人眼脉络膜黑色素瘤OCM-1细胞的机制 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】研究-70℃反复冻融体外杀伤人眼脉络膜黑色素瘤细胞系OCM-1的机制。【方法】OCM-1细胞反复冻融后，用噻唑蓝比色法及克隆形成实验检测细胞的杀伤情况和细胞生长活力，免疫组织化学方法检测细胞P16表达的变化，电镜及共聚焦显微镜观察冻融后细胞凋亡和坏死的形态学改变，并测定细胞的凋亡比率，流式细胞仪检测冻融前后细胞CD40表达比率及细胞膜电位的变化。【结果】噻唑蓝比色法检测及克隆形成实验显示，冻融可以直接杀伤OCM-1细胞，且残存的细胞生长能力也明显受抑制。免疫细胞化学染色见对照组的细胞呈P16阳性，
刘斌 +4 more
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, 2002 Degradation of indole by an indole degrading methanogenic consortium enriched from sewage sludge proceeded through a two step hydroxylation pathway yielding oxindole and isatin.
Fan, Y, Gu, J, Qiu, W, Wang, Y
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Methods in Ecology and Evolution, Volume 14, Issue 7, Page 1732-1746, July 2023., 2023 Abstract Our knowledge on the responses of permafrost ecosystems to climate warming is critical for assessing the direction and magnitude of permafrost carbon‐climate feedback. However, most of the previous experiments have only been able to warm the air and surface soil, with limited effects on the permafrost temperature.
Yuxuan Bai +8 more
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Application status of steel slag asphalt mixture [PDF]
, 2020 总结了钢渣的物理性质、化学成分及矿物相组成; 分析了影响钢渣体积安定性的因素及其改善措施; 探讨了钢渣沥青混合料的配合比设计方法; 分析了钢渣沥青混合料的路用性能(高温稳定性、低温抗裂性、水稳定性、抗疲劳性、体积安定性、抗滑性)及其功能特性(导电性与微波加热); 研究了钢渣沥青混合料的生态、社会及经济效益; 介绍了国内外的工程应用。研究结果表明:钢渣可用于沥青混合料,且应为陈化半年以上的转炉钢渣或电炉钢渣; 钢渣的物理力学性能优良,而化学成分及矿物相组成受炼钢工艺影响有所区别 ...
Gao J. +9 more
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Study on evolution law of mechanical properties of coal samples subjected to freezing and freeze-thaw cycles of liquid nitrogen [PDF]
, 2023 The permeability of coal reservoir is generally low in China, how to effectively improve the permeability of coal reservoir is a key and difficult point of coalbed methane exploitation, Liquid nitrogen fracturing technology as a kind waterless fracturing
Dongming ZHANG +6 more
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Stability of IgE in Postmortem Plasma and Hemolyzed Samples [PDF]
, 2021 Objective To investigate the stability of IgE in postmortem plasma and hemolyzed samples under different storage conditions and freezing-thawing. Methods Thirty nine cardiac blood samples were collected from non-frozen corpses with the postmortem ...
WANG Tian-qi, WANG Tian-fang, JIA Yu-qing, et al
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Zhongguo youzhi, 2023 为提高水代法提取油茶籽油的提油率，将油茶籽经过脱壳、粉碎、过筛、加水过胶体磨、离心分离得到乳状液，经冻融破乳后离心分离得油茶籽油。以提油率为指标，探究了加热冻融、乙醇冻融、调pH冻融、加盐冻融等冻融破乳方法对水代法提取油茶籽油的影响，并通过共聚焦激光扫描显微镜对破乳前后乳状液的微观结构进行了观察。结果表明：乙醇冻融和加盐冻融使油茶籽的提油率降低；调pH可提高提油率，在pH为8时提油率为92.17%；加热冻融法是最佳的冻融破乳方法，在加热温度60 ℃、加热时间20 min条件下，提油率最高，达到92.57%
王亚娟，范亚苇，李静，邓泽元 WANG Yajuan, FAN Yawei, LI Jing, DENG Zeyuan
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Response of Soil Respiration to Storage Irrigation and Freeze-thaw Cycles in Extremely Arid Areas [PDF]
, 2022 [Objective] The effects of water storage irrigation and seasonal freezing and thawing on soil respiration were determined in order to improve the utilization efficiency of irrigation water resources, and save irrigation water resources at Shiyang River ...
Gao Yayu +3 more
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Zhongguo youzhi, 2023 为探究大豆分离蛋白（SPI）及其糖基化产物凝胶在不同温度冻融过程中的变化情况，采用SPI和SPI-葡聚糖（分子质量分别为10、40、70 kDa）轭合物制备凝胶，对其进行-18、-80 ℃冻融处理，测定冻融处理前后凝胶的表面疏水性、总游离巯基含量、凝胶网络结构、凝胶强度和持水力，研究冻融条件对SPI及其糖基化轭合物凝胶理化性质的影响。结果表明：与冻融前比较，-18、-80 ℃冻融处理后SPI凝胶表面疏水性显著增加，总游离巯基含量降低，凝胶网络结构被破坏，凝胶强度总体降低，持水力升高。与冻融前比较，SPI-
罗裕旻1,马钱1，徐子力1，王薇1，张清1，2 LUO Yumin1, MA Qian1, XU Zili1, WANG Wei1, ZHANG Qing1,2
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Mesostructure and strength characteristics of granite under freeze-thaw cycles based on CT scanning [PDF]
, 2023 Objective With the rapid increase in construction projects in the western regions in recent years, the impact of seasonal freeze-thaw cycles in the high-altitude areas of western China has become more pronounced.
CHEN Liang +8 more
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