深低温反复冻融杀伤人眼脉络膜黑色素瘤OCM-1细胞的机制 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】研究-70℃反复冻融体外杀伤人眼脉络膜黑色素瘤细胞系OCM-1的机制。【方法】OCM-1细胞反复冻融后，用噻唑蓝比色法及克隆形成实验检测细胞的杀伤情况和细胞生长活力，免疫组织化学方法检测细胞P16表达的变化，电镜及共聚焦显微镜观察冻融后细胞凋亡和坏死的形态学改变，并测定细胞的凋亡比率，流式细胞仪检测冻融前后细胞CD40表达比率及细胞膜电位的变化。【结果】噻唑蓝比色法检测及克隆形成实验显示，冻融可以直接杀伤OCM-1细胞，且残存的细胞生长能力也明显受抑制。免疫细胞化学染色见对照组的细胞呈P16阳性，
刘斌 +4 more
doaj +2 more sources
, 2002 Degradation of indole by an indole degrading methanogenic consortium enriched from sewage sludge proceeded through a two step hydroxylation pathway yielding oxindole and isatin.
Fan, Y, Gu, J, Qiu, W, Wang, Y
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Application status of steel slag asphalt mixture [PDF]
, 2020 总结了钢渣的物理性质、化学成分及矿物相组成; 分析了影响钢渣体积安定性的因素及其改善措施; 探讨了钢渣沥青混合料的配合比设计方法; 分析了钢渣沥青混合料的路用性能(高温稳定性、低温抗裂性、水稳定性、抗疲劳性、体积安定性、抗滑性)及其功能特性(导电性与微波加热); 研究了钢渣沥青混合料的生态、社会及经济效益; 介绍了国内外的工程应用。研究结果表明:钢渣可用于沥青混合料,且应为陈化半年以上的转炉钢渣或电炉钢渣; 钢渣的物理力学性能优良,而化学成分及矿物相组成受炼钢工艺影响有所区别 ...
Gao J. +9 more
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Study on evolution law of mechanical properties of coal samples subjected to freezing and freeze-thaw cycles of liquid nitrogen [PDF]
, 2023 The permeability of coal reservoir is generally low in China, how to effectively improve the permeability of coal reservoir is a key and difficult point of coalbed methane exploitation, Liquid nitrogen fracturing technology as a kind waterless fracturing
Dongming ZHANG +6 more
core +1 more source
Stability of IgE in Postmortem Plasma and Hemolyzed Samples [PDF]
, 2021 Objective To investigate the stability of IgE in postmortem plasma and hemolyzed samples under different storage conditions and freezing-thawing. Methods Thirty nine cardiac blood samples were collected from non-frozen corpses with the postmortem ...
WANG Tian-qi, WANG Tian-fang, JIA Yu-qing, et al
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Response of Soil Respiration to Storage Irrigation and Freeze-thaw Cycles in Extremely Arid Areas [PDF]
, 2022 [Objective] The effects of water storage irrigation and seasonal freezing and thawing on soil respiration were determined in order to improve the utilization efficiency of irrigation water resources, and save irrigation water resources at Shiyang River ...
Gao Yayu +3 more
core +1 more source
Mesostructure and strength characteristics of granite under freeze-thaw cycles based on CT scanning [PDF]
, 2023 Objective With the rapid increase in construction projects in the western regions in recent years, the impact of seasonal freeze-thaw cycles in the high-altitude areas of western China has become more pronounced.
CHEN Liang +8 more
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Durability and service life prediction of reinforced concrete structures [PDF]
, 2015 This paper presents some durability and service life models for reinforced concrete structures with regard to chloride ingress, carbonation and frost attack.
Boubitsas, Dimitrios +2 more
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Soil Particle Settlement Sorting and Distribution Characteristics of Carbon, Nitrogen and Salt During Gradual Thawing [PDF]
, 2023 [Objective] The classification of sediment particles and the accompanying distribution characteristics of carbon, nitrogen and salt in the process of soil thawing layer by layer under snowmelt runoff scenario were analyzed in order to identify the ...
Hu Yaxian +5 more
core +1 more source
Effects of Winter Irrigation on Soil Water-heat-salt Migration and Crop Growth During Freezing-Thawing Period in Qingtongxia Irrigation District of Ningxia [PDF]
, 2023 [Objective] The effects of winter irrigation on soil water-heat-salt migration and crop growth during the freezing-thawing period were analyzed in order to provide a scientific basis for controlling soil salinization, and to ensure food security ...
Han Yuping +4 more
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