Results 1 to 10 of about 6,472 (160)

对苯二甲酸二甲酯及其异构体的好氧微生物降解 [PDF]

open access: yes, 2004
Five morphologically distinct bacteria isolated from mangrove sediment by enrichment culture, were identified and tested for their ability to degrade dimethyl terephthalate (DMT) and its isomer dimethyl isophthalate (DMI). DMT at a concentration of 98 mg/
Gu, J, Li, J
core   +8 more sources

Identification of Bacterial Strain Isolated from Life Nutrition Liquid(一株分离自“生命营养液”细菌菌株的分类鉴定) [PDF]

open access: yesShipin kexue jishu xuebao, 2016
The bacterial strain CICC 10820 isolated from life nutrition liquid was identified via 16S rRNA and tpi gene sequences analysis. According to the result, strain CICC 10820 was identified as Clostridium tertium.
ZHANG Xin(张欣)   +5 more
doaj   +1 more source

Isolation and Identification of Pediococcus lactis in Human Intestinal Tract and Its Performance Analysis of Fermented Goji Berry Juice [PDF]

open access: yes, 2023
In order to obtain candidate strains with strong colonization ability and excellent fermentation performance of goji berry juice, 3 strains of lactic acid bacteria were isolated and screened from human intestinal tract.
Haitian FANG   +6 more
core   +1 more source

北美水貂源沙门氏菌的分离鉴定及耐药性检测

open access: yes野生动物学报, 2021
为调查秦皇岛地区北美水貂沙门氏菌的检出率及耐药情况,对65份水貂样品中的细菌进行分离纯化,采用细菌的鉴定培养、革兰氏染色、生化鉴定和16S rDNA鉴定分离菌,采用小鼠致病性试验研究分离菌株的致病性,采用KB纸片法检测分离菌的耐药性。结果显示,经鉴定共计分离出9株沙门氏菌,分离率1385%(9/65);9株沙门氏菌均可致小鼠发病死亡,死亡率20%—100%;9株沙门氏菌分别对5—11种抗生素药物耐药,氟苯尼考、替米考星、磺胺间甲氧嘧啶、磺胺二甲氧嘧啶和复方新诺明的耐药率高达100%,土霉素敏感率最高 ...
刘勃兴 赵安奇 王利丽 张雪佳 郭蕊 丁家麟 史秋梅 张志强
doaj  

Determination of glucosinolates by liquid chromatography-mass spectrometry(液相色谱-质谱联用分离、鉴定硫代葡萄糖苷)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2004
从油菜籽中提取硫苷,经弱阴离子交换剂DEAE Sephadex A-25做预处理,得到硫苷提取液.经反相离子对色谱确定分离条件,釆用液相色谱-电喷雾接口-质谱联用,再经紫外扫描进一步确证,同时分离鉴定了油菜籽中7种硫苷.
YUANLi-feng(袁丽凤)   +2 more
doaj   +1 more source

酒泉市狐狸消化道蛔虫分子鉴定及cox1基因序列分析

open access: yes野生动物学报, 2020
为了确定从酒泉市某养殖场北极狐(Alopex lagopus)肠道分离蛔虫的种类,本次研究通过形态学和分子生物学方法对分离的虫株进行鉴定。在对6条分离于北极狐小肠蛔虫进行形态学鉴定的基础上,应用PCR技术对分离株的线粒体cox1基因部分序列进行扩增、测序与分析。形态学鉴定结果表明6条蛔虫与弓首蛔虫形态学特征基本一致;测序结果显示,6个虫株cox1基因序列大小为418 bp,其序列同源性为994%—1000%,与GenBank收录的狮弓蛔虫分离株同源性最高,为994%—997 ...
张建明
doaj  

Optimization of mixed fermentation technology of Lactobacillus helveticus andLactobacillus rhamnosus based on high viable count [PDF]

open access: yes, 2016
以瑞士乳杆菌和鼠李糖乳杆菌混合发酵培养,利用两菌株细胞代谢的差异,以静置发酵24 h后得到的活菌数目为指标,通过单因素实验和正交实验,研究了瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最适发酵条件。结果表明,瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最优条件为:发酵温度37℃,初始pH=6.8,接种量6%,瑞士乳杆菌:鼠李糖乳杆菌=1∶2,瑞士乳杆菌优先接种3 h。最终得到乳酸菌总活菌数为7.2×10~9m L~(-1)。与在相同条件下单独发酵的瑞士乳杆菌和鼠李糖乳杆菌活菌数相比,分别提高了1∶8倍和10.
李敬龙   +3 more
core   +1 more source

Isolation,identification and gas generation of spoilage microorganisms from swollen braised brisket with potato [PDF]

open access: yes, 2020
In order to solve the problem of bag expanding of braised brisket with potato,the microorganism of expanding bag was separated,purified,identified and gas production characteristics were studied.Seven dominant strains were obtained.Morphology observation,
CHEN Hui   +7 more
core   +1 more source

Isolation and identification of algicidal substances produced by Brevibacterium sp. BS01 and algicidal mechanism of algicidal compound against Alexandrium tamarense [PDF]

open access: yes, 2012
赤潮的控制与防治对于保证海洋环境安全和海洋生态健康是至关重要的。本论文主要研究了海洋杀藻放线菌BS01的种属关系;根据BS01杀藻活性物质的基本理化性质,分离纯化得到一个具有杀藻活性的化合物;同时探讨了塔玛亚历山大藻在BS01胞外分泌物胁迫下生理生化水平的响应;考察藻细胞死亡过程中细胞形态变化;氧化压力下藻细胞光合系统损伤情况;综合运用real-timePCR技术研究了藻细胞在杀藻活性物质作用下靶功能基因psbA、psbD、hsp、cox和cob转录水平变化。主要研究结果如下: (1 ...
安新丽
core  

化感物质的抑藻作用研究、应用及生态安全性评价 [PDF]

open access: yes, 2012
水体富营养化日益严重,水华频繁爆发,如何有效控制水华,治理富营养化水体是目前水环境领域的研究热点和前沿。目前湖泊藻类控制技术主要有:物理方法、化学法、生物法,但是这些方法都有其固有的缺点。利用植物化感作用抑制有害藻类生长具有廉价、生态安全等优点近年来备受关注。化感作用就是生物体产生的生物活性物质即化感物质在生物体之间传递信息并导致生物体相互作用。归纳了国内外不同生活型水生植物化感作用研究的主要成果(包括已报道的抑藻水生植物种类、已从水生植物体内和种植水中分离鉴定得到的化感物质 ...
吴振斌, 王红强
core  

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