Results 11 to 20 of about 3,161 (182)
ABSTRACT The purpose of this study is to determine the relationship between productivity and socioeconomic characteristics, as well as the degree to which job satisfaction and engagement can strengthen or moderate this association. With a focus on the mediating roles of job satisfaction and job engagement, this study examines the complex relationship ...
Abdullah Al Masud +6 more
wiley +1 more source
Abstract Urgency to save species from extinction has prompted increased investment in law enforcement in protected areas. To date, such law enforcement has largely focused on increasing costs and reducing opportunities for offending. However, these resource‐intensive approaches are not always effective and can contribute to conflict between people and ...
Freya A. V. St. John +3 more
wiley +1 more source
荧光PCR探针熔解曲线法检测结核分枝杆菌耐利福平突变研究 [PDF]
目的对荧光PCR熔解曲线法检测结核分枝杆菌耐利福平突变方法进行临床研究,评价检测能力及在国境口岸的应用价值。方法应用荧光PCR熔解曲线法检测结核病人临床分离结核分枝杆菌,以传统药物敏感性试验为标准,获得该方法的灵敏性、特异性。结果对347例结核病人临床分离培养样本,传统药物敏感性试验检出271例利福平敏感标本,76例利福平耐药标本。荧光PCR熔解曲线法检出269例利福平敏感标本,78例利福平耐药标本,灵敏性为93.42%,特异性为97.42%,符合率为96.54%。结论荧光PCR熔解曲线法检测速度快速 ...
李庆阁 +4 more
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Abstract Wildlife tourist attractions (WTAs) can permit interactions between humans and wildlife that are detrimental to the animals’ welfare and species conservation. Social media portraying human–wildlife interactions could affect people's perceptions of their acceptability and desirability or stimulate demand for detrimental practices.
T. P. Moorhouse, A. Elwin, N. C. D'Cruze
wiley +1 more source
Selective Isolation of Rare Actinomycetes from Rhizosphere Soil and Identification of Two Novel Strains [PDF]
放线菌的次级代谢产物是重要的药物资源,目前已发现的数万种天然抗生素中约70%是由放线菌所产生的。近年来,由于从链霉菌中不断分离得到重复的活性化合物,继而人们逐渐将目光转向稀有放线菌。稀有放线菌种类多样,并且具有某些不同于链霉菌的特殊代谢途径,因而更有可能产生结构新颖、生物活性独特的化合物,具有极大开发潜力。 本文以采自厦门翔安香山、南京中山植物园和玄武湖公园的植物根际土壤作为分离源,采用聚乳酸-明胶(PLA-G)培养基和海藻糖-脯氨酸(YIM212)培养基进行稀有放线菌的选择性分离 ...
黎丹
core
Finding floral and faunal species richness optima among active fire regimes
Abstract Changing fire regimes have important implications for biodiversity and challenge traditional conservation approaches that rely on historical conditions as proxies for ecological integrity. This historical‐centric approach becomes increasingly tenuous under climate change, necessitating direct tests of environmental impacts on biodiversity.
Zachary L. Steel +8 more
wiley +1 more source
目的建立利福平耐药基因rpoB高通量突变文库。方法通过同源重组和L5 attB噬菌体整合位点交换构建耻垢分枝杆菌ΔrpoB attB::rpoB菌株;基于L5 attB质粒交换系统和抗性选择培养基,挑取48个克隆以验证质粒替换的效率;针对利福平耐药决定区(RRDR)区域内以每3个碱基为单位设计简并性引物,通过PCR扩增获得该区域81个碱基的全覆盖式突变文库;文库片段与载体进行无缝克隆转化到大肠杆菌中形成大肠杆菌突变文库;基于质粒交换原理,将突变质粒库转化至耻垢分枝杆菌ΔrpoB attB ...
陈勇, 冯思源, 徐霖, 田国宝
doaj
ABSTRACT This study examines the ability of households to maintain economic welfare and the threshold at which it occurs. We use the Mobile Financial Service Survey (MFSS) data comprising a probability sampling of all Ghanaian households for the econometric analysis.
Alhassan A‐. W. Karakara +2 more
wiley +1 more source
患者,女,46岁,因"全身酸痛、少尿、恶心呕吐3 d"于2叭3年9月15日入院。既往于1995年诊断为腰椎结核,曾行三联抗结核治疗(利福平、异烟肼、乙胺丁醇)10月余,后未复查;2013年8月31日再次因"腰痛半月"诊断为腰椎结核(L2-3),行抗结核治疗(利福平0.45 g、乙胺丁醇0.75 g、异烟肼0.3 g)后即刻出现全身寒颤、恶心呕吐,遂停利福平数天后调整剂量利福平0.30 g再次口服一次,仍出现上述症状即停用利福平,于9月9日自动出院;9月11日自行口服利福喷丁0.45 ...
罗先荣, 彭家清
doaj
从广东省以及海南省等地区养殖的患病罗非鱼体内分离、收集到多株致病菌,经生化分析和分子生物学鉴定,均为无乳链球菌。对这些菌株分别进行了耐药谱测定、分子分型试验以及分子血清型分析。药敏试验结果表明,2007—2010年分离到的无乳链球菌耐药谱基本相似;多位点可变数目串联重复序列分析(MLVA)试验中,选择5个高变异指数的可变数目重复位点(VNTR)进行分子分型,结果表明,所有鱼源无乳链球菌菌株为同一MLVA型,而作为对照的牛源无乳链球菌则明显不同;为了对这些菌株进一步分型 ...
张德锋 +5 more
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