Results 21 to 30 of about 3,513 (162)

分枝杆菌利福平耐药基因rpoB高通量突变文库的构建

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2023
目的建立利福平耐药基因rpoB高通量突变文库。方法通过同源重组和L5 attB噬菌体整合位点交换构建耻垢分枝杆菌ΔrpoB attB::rpoB菌株;基于L5 attB质粒交换系统和抗性选择培养基,挑取48个克隆以验证质粒替换的效率;针对利福平耐药决定区(RRDR)区域内以每3个碱基为单位设计简并性引物,通过PCR扩增获得该区域81个碱基的全覆盖式突变文库;文库片段与载体进行无缝克隆转化到大肠杆菌中形成大肠杆菌突变文库;基于质粒交换原理,将突变质粒库转化至耻垢分枝杆菌ΔrpoB attB ...
陈勇, 冯思源, 徐霖, 田国宝
doaj  

Influences on cannabis policy design and adaptation in the American states

open access: yesPolicy Studies Journal, Volume 53, Issue 3, Page 723-746, August 2025.
Abstract Despite federal prohibitions, three‐quarters of states had implemented medical marijuana laws (MML) and one‐third had implemented recreational cannabis laws (RCL) by 2020. State policy designs varied considerably, and we focus on policy development and maturation in policy implementation, examining factors influencing design features of policy
Grant W. Neeley   +1 more
wiley   +1 more source

用化学降解动力学方法预测本院利福平滴眼液保存期

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 1985
利福平滴眼液对热不稳定随着温度升高和/或时间延长其含量逐渐下降。为了探讨本院制的利福平滴眼液在室温条件下的保存期,我们采用化学降解动力学(恒温加速破坏)法进行预测。
葛筑珍
doaj  

‘How Does Living in Institution Impact Young Lives?’—Insights From Young People in Ci'aiyuan

open access: yesChildren &Society, Volume 39, Issue 4, Page 760-769, July 2025.
ABSTRACT Institutional care often carries a negative reputation due to its perceived adverse effects on children and young people. However, it remains a significant form of alternative care in China. In Xiangxi, childcare institutions, known as ci'aiyuan, have been further expanding by involving wider populations in recent years.
Shian Yin
wiley   +1 more source

What Do People Want From a Welfare System? Conjoint Survey Evidence From UK Adults

open access: yesPoverty &Public Policy, Volume 17, Issue 2, June 2025.
ABSTRACT What do people want from a welfare system? Previous research has suggested a list of desiderata, such as that the system: reduces poverty; reduces inequality; improves mental and physical health; costs little; and rewards only the deserving. How do these different features trade off against one another to determine overall desirability?
Daniel Nettle   +3 more
wiley   +1 more source

Conceptions of global competence among local university students in Hong Kong: A prototype study

open access: yesAsian Journal of Social Psychology, Volume 28, Issue 2, June 2025.
Abstract Global competence has gained momentum in international policy and scholarly discourses. As a multidimensional construct, its conceptualisation has been ambiguous. So far, the OECD Global Competence Framework has been the most updated and comprehensive framework for understanding global competence.
Ji Ying, Dala Duo
wiley   +1 more source

噬菌体裂解法检测结核杆菌药物敏感性的研究

open access: yesZhongguo shiyan zhenduanxue, 2013
目的利用结核分支杆菌噬菌体裂解法检测结核杆菌对利福平、异烟肼、链霉素敏感性。方法选用传统方法判定为利福平、异烟肼、链霉素的耐药或敏感菌株,采用噬菌体裂解法检测其对以上三种药物的敏感性,对比其特异性和敏感度。结果运用噬菌体裂解法检测结果显示,异烟肼药物浓度为1μg/ml时,其灵敏度为87.0%,特异度为93.3%,与传统方法的符合率为92.0%;链霉素药物浓度为4μg/ml时,其灵敏度为96.2%,特异度为92.0%,与传统方法的符合率为92.9%;利福平药物浓度为5μg/ml时,其灵敏度为91.4 ...
于宏波, 宋阳, 齐红, 韩忠波
doaj  

Impact of Contract Farming on Household Income in Senegal

open access: yesPolitics &Policy, Volume 53, Issue 3, June 2025.
ABSTRACT This study assesses the exploratory factors for household participation in contract farming and its relationship with farm income. We use instrumental variable models. Survey data collected under the Agricultural Policy Support Project (PAPA) in 2017 are used. Two results are obtained.
Thierno Thioune   +2 more
wiley   +1 more source

基因芯片技术在分枝杆菌菌种鉴定和结核耐药性检测中的应用及评价

open access: yesZhongguo shiyan zhenduanxue, 2015
目的探讨基因芯片技术在结核病快速诊疗中的应用价值。方法对抗酸染色涂片阳性的肺结核患者痰标本同时进行基因芯片菌种鉴定和BACTEC MGIT 960培养,对结核阳性的样本分别用以上两种方法检测利福平和异烟肼的耐药性。以培养法为参考方法,评价基因芯片法菌种鉴定和耐药检测的敏感度、特异度和符合率。采用Kappa检验分析两种方法的一致性。结果 656例涂片阳性的患者中,两种方法各检出非结核分枝杆菌21例,符合率为100%。耐药性检测发现,基因芯片对利福平的敏感度为87.8%(86/98),特异度为96.0 ...
李晓非   +4 more
doaj  

China's Eco‐Civilisation, Climate Leviathan, and Hobbesian Energy Transition

open access: yesAntipode, Volume 57, Issue 3, Page 830-861, May 2025.
Abstract Scholars have hitherto tended to theorise China's ecological civilisation project either as a form of environmental authoritarianism or as a vision of eco‐socialism. This paper contributes to the conversation by conducting a textual analysis of Chinese scholarly discussions on eco‐civilisation.
David Chen
wiley   +1 more source

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