Results 21 to 30 of about 2,943 (80)
, 2004 【目的】构建前列腺癌患者的噬菌体抗体库,为前列腺癌特异性的人源性抗体Fab片段的筛选及其应用奠定基础。【方法】由前列腺癌患者外周血分离得淋巴细胞,完整提取其总RNA,经RT-PCR及半套式扩增得到免疫球蛋白全部轻链和重链Fd段基因,然后经酶切、纯化、连接等步骤克隆进载体pComb3,并电转化大肠杆菌XL1-Blue,从而构建前列腺癌患者噬菌体抗体库。【结果】成功构建噬菌体抗体Fab片段库,其轻链及重链Fd段与载体DNA的重组率分别为87%、79%,库容为23.2×107,滴度为87×1012mL-1 ...
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No-Carrier-Added Lutetium-177 Separation Technology Status [PDF]
Medical isotopes are the substantial basis of the diagnostic and therapeutic nuclear medicine. There are irreplaceable advantages for utilizing the nuclear medical technology to diagnose and treat the malignant tumors, cardiovascular and cerebrovascular ...
LI Bo1, 2, 3, HU Yingjiang1, 2, 3, WU Jianrong1, 2, 3, WANG Lei1, 2, 3, CHEN Yunming1, 2, 3, ZHANG Jinsong1, 2, 3, LUO Ning1, 2, 3
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Selective thrombosis in tumor vessels induced by fusion proteins tTF- EG3287 Targeting Neuropilin-1 [PDF]
, 2009 选择性栓塞肿瘤组织血管从而诱发肿瘤组织的缺血性坏死是一种很有前景的抗肿瘤策略。利用导向载体构建载体-tTF融合蛋白，将截断的组织因子tTF选择性靶向于肿瘤组织血管膜表面，可以诱发肿瘤组织血管栓塞，阻断肿瘤营养物质和氧气的供给，引发肿瘤缺血性坏死，发挥抗肿瘤作用。Neuropilin-1(NRP1)是相对分子质量(Mr)为120–130kDa的跨膜糖蛋白，被发现在肿瘤内皮细胞和肿瘤细胞上都有高表达。Neuropilin-1是血管内皮生长因子165(VEGF165)的非酪氨酸激酶受体 ...
何杰
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Zhongshan Daxue xuebao. Yixue kexue ban, 2008 [目的]探讨前列腺特异膜抗原单克隆抗体7E11C5.3介导铼-188和平阳霉素（PYM）导向治疗前列腺癌细胞系LNCaP的体外作用。[方法]以葡聚糖为中介体制备7E11C5.3-PYM偶联物，间接ELISA法测定免疫活性，2,3,5-氯化三苯基四氮唑（TTC）法测定抑菌活性。采用2-巯基乙醇直接还原法制备188Re-7E11C5.3标记物，纸层析法测定标记率和放化纯度，直线回归外推法测定免疫活性分数。四唑盐（MTT）法测定体外细胞毒作用。[结果]7E11C5.3-PYM偶联物中7E11C5 ...
张勇 +5 more
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Current Status of Preparation of Terbium Radionuclides for Theranostic Applications [PDF]
Terbium has four clinically available radionuclides for nuclear medicine applications, 149Tb, 152Tb, 155Tb, and 161Tb. Their unique decay properties and identical chemistry make it possible to prepare radiopharmaceuticals with same ligand, thus enabling ...
Hongyu LI, Jin DU, Kai WEN, Ziyu ZHAO
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The Research Progress of Monoclonal Antibody Labeled with 89Zr [PDF]
Zirconium-89 (89Zr) is a metallic positron-emitting radionuclide. The relatively long half-life and moderate energy making 89Zr especially suitable for the labeling of large biomolecules such as antibodies for radioimmuno-PET (positron emission ...
Fei DUAN, Hongyu LI, Zhanxiong XIAO
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Progress and Prospects of Research and Development ofRadiopharmaceuticals in China [PDF]
The research and development of radiopharmaceuticals is very important for the early diagnosis and treatment of major diseases such as heart, brain, and tumors.
Hua-bei ZHANG, Yu FANG
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Strategies for Innovative Development of Tumor-targeted Theranostic Radiopharmaceuticals [PDF]
Radiopharmaceutical is an essential component of nuclear medicine and have demonstrated significant potential in disease diagnosis and treatment. In recent years, several tumor-targeted theranostic radiopharmaceuticals have been approved for market ...
HUANG Xuhu1, 2, 3, CHEN Huan1, 2, 3, LI Kexin1, 2, 3, WANG Ning1, 2, 3, ZHUANG Jin1, 2, 3, LI Hongyu1, 2, 3, DU Jin2, 3, 4
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Recent Advances and Strategies in the Development of Therapeutic Radiopharmaceuticals [PDF]
Therapeutic radiopharmaceuticals can be employed in the treatment of various tumor diseases，and their global market share is likely to witness an explosive growth in the recent decade.
Hao CHEN +8 more
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Status and Prospect of Radiolabeled Small Molecule Inhibitors as Tumor Imaging Agents Targeting FAP [PDF]
: Fibroblast activation protein (FAP) exists in tumor stromal fibroblasts and is a significant target for tumor diagnosis and treatment recently. Among the radioactive FAP-targeted tumor imaging agents, small-molecule tracers have received the most ...
HAN Peiwen1, RUAN Qing1, 2, ZHANG Junbo1
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