聚合酶链反应(PCR)样品中的Cl^-、引物等小分子对单链构象多态性一毛细管电泳分析有重要影响。本文用毛细管电泳一间接紫外法测定了PCR样品中的Cl^-浓度，并对乙醇沉淀法和试剂盒纯化法用于PCR样品的纯化效果进行比较，在此基础上研究了Cl^-浓度、引物及其二聚体在SSCP-CE分析中的影响。结果表明 ...
杨青, 石先哲, 许国旺, 赵春霞
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[Development and practice of an interactive chromatography learning tool for beginners based on GeoGebra： a case study of plate theory]. [PDF]
Se PuZhang YH +5 more
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[Performance of Plasma Multigene Methylation Testing in Early Diagnosis of Colorectal Cancer]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanSheng N, Guo Y, Hou X, Wang Y, Xu Y.
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K-ras癌基因的点突变在结直肠癌的发生起重要作用。以异丙醇为聚合反应链转移剂，水相法合成特性粘度为O．70×10^-3m^3／kg，分子量为6．5×10^4的低粘度短链线性聚丙烯酰胺。以6％线性聚丙烯酰胺为筛分介质，分离温度27℃，分离电压9kV为电泳条件，建立了检测K-ras基因突变的毛细管电泳．单链构象多态性方法。利用该方法检测36例结直肠癌患者肿瘤组织，发现12例K-ras基因突变。结果表明：该方法具有快速、高灵敏的优点 ...
吕申 +5 more
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Beijing Da Xue Xue Bao Yi Xue BanWei C, Yang Y, Zhao X, Liu X, Jia Y.
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Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiZhao L, Xie H.
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Nan Fang Yi Ke Da Xue Xue BaoZhang M, Sun F, Han Z, Gao Y, Luo Y.
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Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiZhu K +6 more
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Beijing Da Xue Xue Bao Yi Xue BanWang X, Li L, Zhang W, Wang H.
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