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一种海绵细胞的微载体三维培养方法

, 2005
本发明涉及海洋无脊椎动物细胞的培养，具体地说是一种海绵细胞的微载体三维培养方法；1)在改进人工海水中接种微载体和海绵细胞进行培养；改进人工海水的水溶液中包括：Na+，SO42-，HCO3-，K+，Tris HCl， Cl-，Mg2+，Ca2+，SiO32-，Fe3+，Zn2+，C6H5O73-，pH 7.6～8.2，PHA；2) 在海绵细胞培养5～8天后，向上述改进人工海水中添加有机碳源、有机氮源和维生素C，形成新的培养液体系；3)在步骤2)之后，每隔2～3天更换培养液，并添加有机碳源和氮源，维生素C ...
金美芳, 赵权宇, 张 卫, 虞星炬
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[Targeted inhibition of miR-503 upregulates Apelin expression to alleviate myocardial infarction in mice]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Li J, He Y, Bi F, Chen J, Fan Z, Zhang Y, Chen E, Xiao H, Wu Y, Tian H, Zhou Y.   +10 more
europepmc   +1 more source

[Advances in the molecular genetics of nuclear gene mutations causing pediatric mitochondrial cardiomyopathy]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Wang ZW, Wang YQ, Wang CL.
europepmc   +1 more source

[Research advances on the targeted programmed cell death regulatory network for treatment of refractory diabetic wounds]. [PDF]

Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Zhang ZH, He WF.
europepmc   +1 more source

[<i>Qingshen</i> Granules alleviate renal fibrosis in mice with unilateral ureteral obstruction by regulating Akt3-mediated M1 macrophage polarization]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Wang W, Gao Y, Hong X, Chen Y, Cheng M, Zhang L, Dai R, Wang Y.   +7 more
europepmc   +1 more source

[Explanation and interpretation of pediatric blood requirement recommendations in the national health standard "Guideline for pediatric transfusion"]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Huang R, He QN, Hei MY, Yang MH, Zhu XF, Lu J, Xu XJ, Yuan TM, Zhang R, Wang X, Liu JP, Wang J, Shao ZL, Zhao MY, Guo YJ, Wu XY, Chen JR, Chen QR, Guo J, Gui R.   +19 more
europepmc   +1 more source

[Mechanism of <i>Tongxie Yaofang</i> for reducing 5-HT-induced oxidative stress injury in BRL 3A cells: the mediating role of the SREBP-1/CD1 pathway]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Song M, Su H, Xia H, Chen S, Shao L, Jiang Z.   +5 more
europepmc   +1 more source

[A case of <i>Bartonella henselae</i> meningitis characterized by bone marrow hemophagocytosis]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
DU WQ, Liu LJ, Zhang L, Tang YF, Liu LQ, Li XF, Xiao YY.   +6 more
europepmc   +1 more source

[<i>Trillium tschonoskii</i> Maxim saponin protects neurological function in rats with post-stroke cognitive impairment by promoting ER-phagy]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Yang D, Zhao F, He Y, Zhu H, Liu X, Chen X.   +5 more
europepmc   +1 more source

[Effects of glutathione on acute cutaneous photodamage in mice and its mechanism]. [PDF]

Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Wang Q, Zhou L, Deng Y, Qiu ZJ, Wu X, Zhao C.   +5 more
europepmc   +1 more source