Zhongguo shiyan zhenduanxue, 2023 目的 利用微流控芯片恒温扩增技术(LAMP-Disk)建立多种呼吸道病原体快速检测的方法，并对其临床应用的可行性和价值进行评估。方法 设计和筛选针对甲型流感病毒、乙型流感病毒、呼吸道合胞病毒、腺病毒、副流感病毒、鼻病毒和肺炎支原体的LAMP引物对，利用盘式微流控芯片建立快速检测方法。以人工构建的参考品确定LAMP-Disk方法的检出限和分析特异性。收集268份呼吸道感染患儿的咽拭子样本，使用荧光定量PCR(qPCR)法与LAMP-Disk进行平行检测，从灵敏度 ...
池细俤 +4 more
doaj
Epidemiological characteristics of human parainfluenza virus in Pudong New Area， Shanghai， 2017‒2022 [PDF]
ObjectiveTo investigate the epidemiological characteristics of human parainfluenza virus (HPIV) in Pudong New Area, Shanghai, and to provide a basis for the prevention and control of HPIV infections in this area.MethodsA total of 8 180 cases with acute ...
CHU Qiang +5 more
core +1 more source
Zhongguo shiyan zhenduanxue, 2021 目的分析ICU内老年重症肺炎患者呼吸道感染的血清流行病学特征,为临床预防和治疗提供参考依据。方法以2019年1月至2020年1月收治于河南省人民医院ICU的200例老年重症肺炎患者为研究对象,收集患者血清,采用间接免疫荧光法(Indirect immunofluorescence assay,IFA)检测患者血清中9种呼吸道病原体IgM抗体,即肺炎支原体(Mycoplasma pneumoniae,MPn)、肺炎衣原体(Chlamydia pneumoniae,CPn)、嗜肺军团菌(Legionella
裴永菊 +4 more
doaj
Adult-onset Still's disease complicating late pregnancy: a case report and literature review [PDF]
Adult-onset Still's disease (AOSD) is a rare autoinflammatory disorder characterized by nonspecific clinical manifestations and the absence of definitive laboratory markers.
CHEN Kai +3 more
core +1 more source
Correlation between Chlamydia Pneumoniae IgG Positive in Lung Cancer Patients and Cytokines Related to Radiation-induced Pulmonary Lesion [PDF]
, 2011 Background and objective There exsits intimate relationship between infection with chlamydia pneumoniae (Cpn) and lung cancer incidence. But few studies have been reported about radiation-induced pulmonary lesion in lung cancer patients infected with Cpn.
Daoan ZHOU +3 more
core
Zhongguo shiyan zhenduanxue, 2020 在日常生活中,呼吸道感染属于常发疾病之一,主要临床症状为发热,如没有得到及时治疗,会导致患者的神经系统等受到损害[1]。这种疾病的病原学较为复杂,同时具备季节性流感等特征。随着医疗水平不断提高,呼吸道病原体IgM抗体检测在患者的呼吸道感染中具有较大的占比,使用范围也不断的扩大,诊断过程中主要是依靠间接免疫荧光法,对较为常见的呼吸道非细菌病原体,例如 ...
司序, 杨裕颖, 陈雷
doaj
An analysis of epidemiological characteristics of influenza-like illness in Hongkou District， Shanghai from 2015 to 2024 [PDF]
ObjectiveTo explore the epidemic levels and epidemiological characteristics of influenza-like illness (ILI) in Hongkou District of Shanghai, to track the trends in virus mutations, so as to offer a scientific foundation for precisely predicting influenza
LI Tong +4 more
core +1 more source
Influenza virus infection of influenza-like illness at a sentinel hospital in Baoshan District of Shanghai from the monitoring year of 2015 to 2023 [PDF]
ObjectiveTo investigate the influenza virus infection status of influenza-like illness (ILI) at a sentinel hospital in Baoshan District of Shanghai, to explore the seasonal patterns of influenza, so as to provide a scientific basis for influenza ...
DOU Wenxia +5 more
core +1 more source
, 2006 [目的]利用低密度基因芯片方法对人乳头瘤病毒（HPV）进行分型检测,建立一种经济实用的临床HPV感染的基因型检测方法.[方法]利用低密度基因芯片方法对145例阴道镜门诊病人的宫颈拭子标本进行HPV-DNA检测并分型.[结果]被检人群中共检测出57例HPV阳性,88例HPV阴性,HPV的感染率为39.3%,阳性标本中检测出12种高危型别,2种低危型别.其中单型感染51例（89%）,混合感染6例（11%）,16,31,18,58型检出率较高.[结论]低密度基因芯片是一种快速、简便、特异性强 ...
何蕴韶 +4 more
core
Exploring the mechanism of matrine on lung inflammation in mice infected with respiratory syncytial virus by regulating PI3K/AKT signaling pathway [PDF]
Objective To explore the protective mechanism of matrine against respiratory syncytial virus (RSV) infection in mice based on the PI3K/AKT signaling pathway.
DU Mingwei +8 more
core +1 more source