本发明涉及生物化工中对酶进行固定化的工艺,具体地说是载体 ZH-HA固定化粪产碱杆菌青霉素G酰化酶的方法,固定化粪产碱杆菌青霉素G酰化酶的载体为ZH-HA,于缓冲溶液体系中,以ZH-HA进行固定化时,酶浓度为为180-240mg/ml,固定化时间12-18h,缓冲溶液pH 7.5 -10.0,反应体系中盐的1.0-1.5M,载体与缓冲溶液的重量体积比例为1g 载体:4-6ml缓冲溶液。本发明固定化酶的温度、pH稳定性都比自由酶有了改善。经过连续多批水解青霉素G钾盐,固定化粪产碱杆菌青霉素G ...
许国旺, 孙 健, 袁中一
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Li SJ, Li Z, Tang ZX, Lyu M, Wang LT.
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底泥是湖泊重要的内源污染来源,关于底泥污染控制的研究近年来受到广泛重视。目前对底泥污染的控制技术主要包括原位处理技术和异位处理技术。原位处理技术主要包括钝化、覆盖、生物修复等。钝化技术是一种高效的湖泊内源污染控制技术,它通过抑制内源营养盐的释放来控制水体营养盐含量。文章综述了底泥原位钝化技术的研究及其应用情况;分析了原位钝化技术的特点及主要功能;介绍了常用底泥钝化剂的应用情况以及新型钝化剂的研究进展 ...
吴振斌, 贾陈蓉, 梁威, 吴春芸
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