Results 51 to 60 of about 897 (148)
Development of Real-time PCR Genotyping [PDF]
, 2004 本论文围绕实时PCR基因分型的探针基因分型和引物基因分型两个关键技术展开了研究，研究工作主要包括新型荧光双链置换探针实时PCR基因分型技术的研究，以及新型硫代磷酸化修饰的引物结合高保真DNA聚合酶的等位基因特异扩增的基因分型技术的研究。 第一部分，提出了新型荧光双链置换探针实时PCR基因分型的新技术，以β-地中海贫血中国地区常见的5种突变为研究对象，建立了新型荧光双链置换探针实时PCR基因分型的检测体系，系统考察了新型荧光双链置换探针检测的灵敏度和特异性、以及影响探针灵敏度和特异性的关键因素 ...
程金平
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, 2002 摘 要:【目的】用三七总皂甙(total Panax notoginseng saponins)在体外定向诱导SD 青年鼠骨髓间质干细胞(mesenchymal stem cell , rMSC)分化为神经元样细胞。【方法】用低糖DMEM 冲洗骨髓腔, 收集骨髓细胞悬液, 接种在塑料培养瓶中。 经体外扩增、纯化, 选用第5 代后的间质干细胞进行诱导分化。用10 μg/ L bFGF 预诱导24 h, 更换成含三七总皂甙的无血清 培养基DMEM 诱导间质干细胞分化为神经元样细胞。用免疫组化SABC ...
撒亚莲, 李海标
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Research Progress on the Effect of the Interaction Between Calcium and Iron on Their Absorption in the Intestine [PDF]
Calcium and iron are essential metal elements for human health, as they play important roles in various metabolic processes such as bone formation and oxygen transport.
LI Yi, CHENG Yongqiang, TANG Ning
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Zhongguo shiyan zhenduanxue, 2012 地中海贫血是一组遗传性溶血性贫血,其特点是由于珠蛋白基因的缺陷使血红蛋白中的珠蛋白肽链有一种或几种合成减少或不能合成,导致血红蛋白的组成成分改变,本组疾病的临床症状轻重不一,大多表现为慢性进行性溶血性贫血。在广西,每年约有17万新生儿为地中海贫血基因携带者 ...
刘铁牛 +4 more
doaj
Thalassemia Reports, 2012 Nonsense-mediated mRNA decay (NMD) is a surveillance system to prevent the synthesis of non-functional proteins. In β-thalassemia, NMD may have a role in clinical outcome.
Maria Concetta Renda +11 more
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Detection of complex hemoglobinopathies: recommendations on screening and DNA testing
Thalassemia Reports, 2011 The following recommendations should be taken into account during the evaluation and elucidation of the complex hemoglobinopathies: a) in complex hemoglobinopathies performing DNA studies on all family members might be essential; b) complex gene-gene ...
E. Baysal
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Fast Magnetic Susceptibility Reconstruction Using L0 Norm Gradient Minimization [PDF]
, 2015 在磁共振成像领域，定量磁化率成像技术受到越来越多的关注。磁化率作为组织自身固有的属性，能够提供一种显著的对比度机制和比相位更能反映组织的组成成分。每个组织都有自己的磁化率值，比如含钙和铁沉淀的组织等。到目前为止，磁化率能够为诊断再生障碍性，阿兹海默病，地中海贫血症，血色素沉着症和帕金森症等疾病提供帮助。 定量磁化率成像一般经过三个步骤，第一步是相位解缠绕，第二步是背景场去除，最后是对得到的局部场进行磁化率反演。其中在磁化率反演过程中，由于偶极子核在锥面区域存在零值 ...
刘伟骏
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红细胞参数的运算公式对缺铁性贫血与β-地中海贫血鉴别诊断价值探讨
Zhongguo shiyan zhenduanxue, 2012 目的探讨红细胞参数的运算公式对缺铁性贫血与β-地中海贫血鉴别诊断的实用价值。方法使用全自动血细胞分析仪对160例缺铁性贫血和172例β-地中海贫血患者的红细胞相关参数进行检测,以文献报道的5个红细胞参数的运算公式作为缺铁性贫血与β-地中海贫血的鉴别指标,分析它们的灵敏度、特异度、假阳性率、假阴性率、阳性预测值、阴性预测值、约登指数、阳性似然比、阴性似然比、对应的ROC曲线以及曲线下面积。结果在诊断IDA时,MI、S&BI、SI、和EI灵敏度在72.5%-81.88%,但特异度较低,在22.09%-26 ...
陶义丰 +4 more
doaj
, 2016 2009-2010 > Academic research: refereed > Publication in refereed journalVersion of ...
Qian, ZM, 李艳伟, 赵晋英
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Fertility assessment in thalassemic men
Thalassemia Reports, 2017 Male infertility in β-thalassemia patients is typically considered to be the consequence of iron deposition in the endocrine glands. Adult male patients with β-thalassemia, on regular blood transfusions, are prone to developing acquired hypogonadism. The
Shahla Ansari +5 more
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