Results 61 to 70 of about 6,787 (165)
Effects of dynamic monitoring of plasma procalcitonin levels in sepsis patients in guiding antibiotic treatment on course of treatment，drug resistance and prognosis [PDF]
"Objective To explore the effects of dynamic monitoring of plasma procalcitonin（PCT）levels in patients with sepsis in guiding antibiotic treatment on course of treatment and drug resistance，and to evaluate its significance in predicting patient prognosis.
SU Li *，ZHANG Ruiying，LI Queque
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Zhongguo shiyan zhenduanxue, 2007 目的探讨K562细胞多重耐药性形成的分子机制。方法采用MTT法分别测定K562及阿霉素耐药的K562细胞对顺铂的耐药程度,采用RT-PCR检测细胞表面MDR、MRP、GST及MGMT分子表达水平,并确定其与耐药性的关系。结果顺铂对K562细胞的抑制率明显高于阿霉素耐药的K562细胞,同时阿霉素耐药的K562细胞的表面MDR、MRP、GST及MGMT的表达明显增高。结论MDR、MRP、GST及MGMT的高表达是K562细胞形成多重耐药性的重要机制之一。
杨冬艳 +5 more
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Zhongguo shiyan zhenduanxue, 2021 目的分析某三级综合性医院重症监护病房(ICU)2019年分离病原菌特征及耐药性,为临床用药和院感防控提供依据。方法收集ICU 2019年送检标本,按标准操作规程进行培养鉴定及药敏试验,采用WHIONET 5.6软件进行数据分析。结果共分离病原菌245株,阳性率16.94%。分离病原菌种类以鲍曼不动杆菌、肺炎克雷伯菌、铜绿假单胞菌、大肠埃希菌和奇异变形菌等为主。药敏结果显示,铜绿假单胞菌对亚胺培南耐药率最高(57.14%),对其它测试药物均低于50%;鲍曼不动杆菌、肺炎克雷伯菌 ...
侯盼飞 +4 more
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Distribution and drug resistance characteristics of Acinetobacter baumannii in the environment of a general hospital in Xuhui District of Shanghai from 2018 to 2023 [PDF]
ObjectiveTo analyze the distribution, drug resistance characteristics, and changing trends of Acinetobacter baumannii (AB) isolated from environmental surfaces and healthcare workers’ hands in a grade Ⅱ level A general hospital in Xuhui District of ...
JIANG Lufang +8 more
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Zhongguo shiyan zhenduanxue, 2011 目的探讨临床分离的多重耐药铜绿假单胞菌耐药性及相关耐药基因。方法采用纸片扩散法进行药敏试验,VITEK 2全自动微生物鉴定及药敏系统对其进行重新鉴定,聚合酶链反应（PCR）扩增oprD2、金属酶基因、氨基糖苷类酶修饰基因。结果 40株铜绿假单胞菌对临床常用的抗生素:厄它培南,亚胺培南（IPM）、头孢他啶（CAZ）、美罗培南、头孢吡肟（PEP）、哌拉西林/他唑巴坦（TZP）、环丙沙星、氨曲南（ATM）、庆大霉素（GEN）、妥布霉素、阿米卡星、多粘菌素耐药率分别为100%、96.2%、92.0%、90.1%、
赵祝香 +4 more
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Chemical constituents from root of Angelica decursiva [PDF]
, 2017 采用HP20大孔吸附树脂、ODS、硅胶柱色谱、Sephadex; LH-20柱色谱和半制备HPLC等色谱分离手段对紫花前胡的化学成分进行研究。结合理化性质及MS,NMR等谱学数据鉴定化合物的结构,从紫花前胡80; %乙醇提取物中分离并鉴定了12个化合物,分别是(9R,10R)-9-acetoxy-8,8-dimethyl-9,10-dihydro-2H,8; H-benzo[1,2-b: 3,4-b']dipyran-2-one-10-yl; ester(1)、补骨脂呋喃香豆精(2)、顺式-3',4 ...
姜鑫 +4 more
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Zhongguo shiyan zhenduanxue, 2011 目的建立一种多重PCR方法,在同一扩增体系内同时检测病原菌及其β-内酰胺类耐药基因,探讨采用多重PCR同时鉴定病原菌及其耐药基因的可行性。方法根据细菌基因序列和对β-内酰胺类抗生素耐药特点,设计一对细菌鉴定通用引物和三对β-内酰胺类耐药基因引物,并在同一扩增体系内行PCR扩增。结果 MRSA和大肠埃希菌及肺炎克雷伯菌的产酶标准菌株的bla mecA、blaTEM、blaSHV和16S-23S rRNA基因间隔区（ISR）多重扩增均为阳性,对本实验室内保存的50株多重耐药的葡萄球菌 ...
黄艳飞, 鲁辛辛, 孙宇峰
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Antibiotic resistance and molecular typing of Campylobacter spp. from diarrheal patients in Baoshan District of Shanghai， 2019‒2022 [PDF]
ObjectiveTo analyze the drug resistance and the molecular typing characteristics through pulsed field gel electrophoresis (PFGE) of Campylobacter spp. isolated from patients with infectious diarrhea in Baoshan District of Shanghai, and to provide a basis
CUI Shiyong +5 more
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Progress and challenge of food microbiological risk assessment in China [PDF]
, 2020 In the recent 10 years, China has carried out the construction of food safety risk assessment system including microbiological risk assessment (MRA) in accordance with the Food Safety Law, and has made significant progress in the work system and ...
Li BAI +5 more
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Zhongguo shiyan zhenduanxue, 2015 鲍曼不动杆菌是医院感染常见机会致病菌之一,由于其具有强大的获得耐药性和克隆传播的能力,多重耐药,泛耐药,甚至全耐药鲍曼不动杆菌呈世界性流行[1],已成为我国院内感染最重要的病原菌之一。随着抗菌药物的广泛应用,多重耐药鲍曼不动杆菌的检出比例逐年增加,已成为临床抗感染治疗的棘手问题[2-4]。为此,本文对2009年-2013年临床分离的鲍曼不动杆菌进行回顾性总结和分析 ...
于清华, 苏维奇, 陈安青
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