Results 51 to 60 of about 3,303 (156)

Gut microbiota dynamics of adult and sub‐adult sika deer during reintroduction 重引入过程中成年和亚成年梅花鹿肠道微生物的动态变化

open access: yesIntegrative Conservation, Volume 3, Issue 3, Page 257-270, September 2024.
Natural training enclosure design schematic. We selected 12 sika deer including six adults and six sub‐adults. Key considerations in the design of the natural training were to divide the training spatial ground into two parts (30 × 30 m small areas for isolation of new individuals and large areas measuring 200 × 600 m for free walking), and the ...
Wentao Zhang   +10 more
wiley   +1 more source

Research on a Reovirus Disease of Cultured Mud Crab in Fujian Province [PDF]

open access: yes, 2007
2004年11月以来,我国南方各省主要青蟹养殖区暴发大规模流行病,短时间即引起大批死亡,采用各种手段也无法阻止病情的蔓延,已造成严重损失。本研究以福建省厦门和漳州两地养殖场的患病青蟹为实验对象,系统深入地展开病原生物研究工作,从病原的分离纯化、人工感染、组织病理观察、生理生化指标监测等方面着手,分析病原生物的类型,观察并诊断病原的传播途径、致病原因以及发生发展过程,主要实验结果如下: 1.比较确立了适当的病原生物分离纯化方法,并从病蟹体内分离出1株对称球形病毒 ...
邹清
core  

Harnessing green microbiology for sustainable water management, agriculture, and energy generation: Current advances and future prospects

open access: yesWorld Water Policy, Volume 10, Issue 3, Page 649-697, August 2024.
Abstract This article presents an in‐depth overview of recent developments and prospects in utilizing green microbiology for sustainable water management. It highlights its vital role across various aspects of water management while underscoring its significance for global development. This article delves deep into microbial processes integral to water
Adenike A. Akinsemolu   +2 more
wiley   +1 more source

Effects of gingipain extract on the biological characteristics of oral squamous cell carcinoma cell HN6 [PDF]

open access: yes
Objective·To observe the effects of gingipain extract on the biological characteristics of oral squamous cell carcinoma cell HN6.Methods·The HN6 cell line was selected, cultivated, and divided into different groups based on the protein concentration of ...
LI Huxiao   +5 more
core   +1 more source

Rapid, easy, sensitive, low‐cost and on‐site detection of environmental DNA and RNA using CRISPR‐Cas13

open access: yesMethods in Ecology and Evolution, Volume 15, Issue 8, Page 1408-1421, August 2024.
Abstract Environmental DNA (eDNA) monitoring of species distribution has become a critical tool in ecology, conservation biology and fisheries for identifying the presence and distribution of diverse organisms, including important, threatened and invasive species.
Jiwei Yang   +4 more
wiley   +1 more source

microRNA-218通过靶向HOXA10抑制肝癌细胞的实验研究

open access: yesZhongguo shiyan zhenduanxue, 2019
微小RNA(microRNA,miRNAs)是一类长约22个核苷酸的单链非编码RNA,通过与靶mRNAs完全或不完全互补配对,导致靶基因降解或抑制其翻译,从而在基因的表达调控中发挥着重要作用。研究表明microRNAs参与了肝癌的发生、发展和侵袭转移的全过程[1,2]但是有关miR-218在肝癌(HCC)的生物学功能仍然未知 ...
肖钟迪, 王雅丽, 付凯
doaj  

环状RNA对心血管疾病的诊断价值

open access: yesZhongguo shiyan zhenduanxue, 2019
近年来随着非编码RNA的不段发现,越来越多的疾病和非编码RNA异常改变相关[1]。非编码RNA占据全部基因的99%以上[2],被认为在调控编码基因方面起到重要作用。随着微小RNA(miRNA),长链非编码RNA(lncRNA)研究的深入证实上述2种非编码RNA参与到包括肿瘤、神经、血液、心血管等疾病的发病和预后过程[3,4]。随后人们又发现更加稳定的非编码RNA-环状RNA,其广泛的存在于有机体内,因为不同于线性RNA,环状结构使其更加稳定的存在于体内 ...
张云飞, 陈晨, 赵惠敏, 刘杨
doaj  

The functional analysis of Caenorhabditis elegans 14-3-3 protein FTT-2 in pharyngeal muscle cells [PDF]

open access: yes, 2012
14-3-3蛋白是一个几乎在所有真核生物中表达的高度保守的蛋白质家族,它们广泛参与了细胞凋亡、细胞周期调控、蛋白跨膜转运、信号转导等重要生命活动的调节进程。众多的研究表明该蛋白家族的表达异常与多种疾病的发生都密切相关。目前对于14-3-3蛋白的结构以及与其他蛋白间的相互作用研究比较多,而对于14-3-3的亚型特异性功能和组织特异性调控的研究工作却很少。本研究以秀丽隐杆线虫为模式生物来研究秀丽隐杆线虫中仅有的两个14-3-3蛋白FTT-2和PAR-5在功能上的差异。通过对构建的FTT-2和PAR ...
余真
core  

cDNA 微阵列和组织微阵列对卵巢上皮性肿瘤基因的表达

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2004
【目的】结合cDNA微阵列和RNA与冰冻组织微阵列原位杂交的方法以寻找新的特异性卵巢癌候选癌基因,为卵巢癌的早期诊断和治疗提供理论依据。【方法】利用cDNA微阵列筛选在所有3种上皮性卵巢肿瘤中(卵巢浆液性交界性肿瘤、卵巢浆液性腺癌和卵巢子宫内膜样腺癌)显示有意义表达的基因,由RNA与冰冻组织微阵列原位杂交证实其结果。【结果】28个基因在>70%卵巢肿瘤中显示出高表达,18个基因在>70%卵巢肿瘤中低表达;干扰素诱导的转膜蛋白1(IITP1)被进一步用于RNA与冰冻组织微阵列原位杂交研究 ...
doaj  

An in-vitro Study on Reversing the Multidrug Resistance of Ovarioma by siRNA Regulated by hTERT Promoter [PDF]

open access: yes, 2010
背景与目的:卵巢癌死亡率居妇科恶性肿瘤之首,化学药物治疗(化疗)耐药是造成患者总体预后不良的主要原因之一。mdr1基因及其表达产物P-gP被认为是引起多药耐药的主要原因。siRNA沉默技术是近年来兴起的一种新型的基因阻断技术。成功应用RNA干扰沉默mdr1逆转耐药采用RNA聚合酶Ⅲ启动子(H1,U6)表达经典的发夹结构RNA,缺乏组织细胞靶向性。本研究选择了具有组织特异性的hTERT启动子介导靶向mdr1的shRNA的表达,以期特异抑制mdr1表达从而逆转卵巢癌多药耐药。 方法: 1 ...
林燕真
core  

Home - About - Disclaimer - Privacy