准确、方便快捷的禽流感病毒亚型鉴定方法对于野鸟流感病毒感染与流行状况的调查监测是十分必要的。本研究参照Phipps L P等人建立的通用引物RT-PCR HA亚型鉴定方法,分别对已知为H1、H2、H3、H4、H6和H11亚型的22株野鸟流感病毒毒株以及6株未知亚型的野鸟流感病毒毒株进行了HA亚型分型的验证性试验。即利用1对通用引物对所有病毒样品进行RT-PCR扩增,将各PCR产物进行核苷酸序列测定并与GenBank上参考毒株序列进行同源性比较,以确定其HA亚型。BLAST分析显示 ...
张苗 +6 more
doaj
[Discrimination of macrotrabecular-massive hepatocellular carcinoma based on fusion of multi-phase contrast-enhanced computed tomography radiomics features]. [PDF]
Zhang Z +7 more
europepmc +1 more source
[High-throughput circular RNA sequencing reveals tumor-specific high expression of hsa_circ_0001900 in Wilms tumor in association with poor prognosis]. [PDF]
Gao Z +9 more
europepmc +1 more source
[Chinese guideline on the diagnosis and treatment of adult primary immune thrombocytopenia (2025)]. [PDF]
Thrombosis and Hemostasis Group +2 more
europepmc +1 more source
[A large-scale method for the enrichment and identification of N-glycopeptides in microscale plasma samples]. [PDF]
Yang XY, Qin WJ.
europepmc +1 more source
[Proteomics analysis of the effect and mechanism of ADSCs on full-thickness skin defects in diabetic rats]. [PDF]
Gu Y, Chen YZ, Wang SY, Song HY, Bai N.
europepmc +1 more source
Sex Identification of Ardeid Birds by Amplifying Sex Gene Sequence
以鹳形目5种鹭科鸟类和1种鹮科鸟类的组织为实验材料,采用PCR方法扩增其性别基因相关片段,探讨鹭科鸟类性别的分子鉴定方法.通过测定3对已知性别的白鹭(Egretta garzetta)、岩鹭(Egretta sacra)和黄嘴白鹭(Egretta eulo-photes)以及12只未知性别的夜鹭(Nycticorax nycticorax)、池鹭(Ardeola bacchus)、白鹭、牛背鹭(Bubulcus ibis)黑脸琵鹭(Platalea m inor)的性别基因CHD或EE0 ...
江杉, 陈小麟
core
[Preface for Special Issue on Advanced Molecular Imprinting Technology and its Cutting-Edge Applications]. [PDF]
Jia Q, Yang KG.
europepmc +1 more source
[Undergraduate innovation training experiment: zooarchaeology by mass spectrometry for species identification of paleontological remnants]. [PDF]
Xu Y, Jiang LY, Yang SS.
europepmc +1 more source

