慢病毒介导shRNA静默CXCR4对Eca109细胞转移特性的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 2010 【目的】 新近研究显示CXCL12/CXCR4通路在食管癌组织中有较高的表达,本研究探讨应用慢病毒介导的RNA干扰技术,有效静默食管癌细胞株Eca109细胞CXCR4的表达,研究CXCL12/CXCR4通路对食管癌Eca109细胞转移能力的影响65377;【方法】 以人CXCR4 mRNA编码序列作为干扰靶点,构建靶向CXCR4的vshRNA表达质粒,另构建不针对任何已知mRNA的阴性对照vshRNA表达质粒,转染相应组别细胞65377;研究分为控制组65380;阴性对照组及静默组65377 ...
doaj
[Helicid alleviates depression-like behavior in rats with chronic unpredictable mild stress through the NCALD/sGC/cGMP/PKG axis]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoDU X +8 more
europepmc +1 more source
[Protective effect and mechanism of TSPAN9-mediated mitocytosis in interleukin-1β-induced rat chondrocyte senescence]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiChen Q, Da W, Luo N, Shen B.
europepmc +1 more source
[Application and advances of exosome-hydrogel system in wound healing]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiQiu Y, Tan Q.
europepmc +1 more source
[Application and research progress of metal-organic framework materials in skin repair]. [PDF]
Sheng Wu Yi Xue Gong Cheng Xue Za ZhiMa S, Wu W, Liu H, Liu M, Xing F.
europepmc +1 more source
[EVA1A overexpression improves non-alcoholic fatty liver disease in mice by regulating lipid metabolism and promoting lipophagy]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoXu J +8 more
europepmc +1 more source
[Research on tissue regeneration and engineered scaffold materials in prevention and treatment of lymphedema]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiYuan S, Deng C.
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2011 【目的】 探讨超顺磁性氧化铁(SPIO)活体动态示踪小型猪骨髓间充质干细胞(MSC)移植治疗心肌梗死的可行性。【方法】 采用密度梯度离心法获取小型猪自体骨髓MSC,用携带绿色荧光蛋白(GFP)基因的慢病毒载体转染MSC,并用SPIO体外标记MSC-GFP。采用经皮球囊封堵法制备急性心肌梗死(AMI)模型,双标记的MSC经冠脉移植入小型猪心肌梗死区域，移植后24 h、3周、8周在MRI下进行活体动态示踪。【结果】 普鲁士蓝染色显示25 µg Fe/mL SPIO与0.8 µL ...
doaj
[Research progress on the mechanisms and optimization strategies of mesenchymal stem cell-derived exosomes in the treatment of liver diseases]. [PDF]
Zhonghua Gan Zang Bing Za ZhiLi XR, Niu B, Zhang LY.
europepmc +1 more source