Results 31 to 40 of about 16,260 (175)

表达降钙素基因相关肽的单纯疱疹病毒Ⅰ型扩增子载体免疫效果观察

open access: yesZhongguo shiyan zhenduanxue, 2006
目的探讨重组pUPO-Δγ134.5-CGRP扩增子对免疫系统的作用。方法用重组扩增子感染BALB/C小鼠,分离血清及淋巴细胞,流式细胞仪法、免疫组化法、LDH法、分别检测外周血CD4、CD8分类、TCR、NK细胞、CTL细胞以及LAK细胞活性杀伤活性。结果重组扩增子明显增强了机体的免疫力。结论该重组扩增子具有增强机体免疫力的作用。
吴军   +4 more
doaj  

Characterization of the expression patterns, transcriptional regulation, antimicrobial mechanisms and immune activities of SpHyastatin from Scylla paramamosain [PDF]

open access: yes, 2015
拟穴青蟹(Scyllaparamamosain),简称青蟹,是我国东南沿海重要的经济养殖蟹类。迄今已有报道抗菌肽在青蟹抵抗海洋环境中病原微生物的入侵感染方面具有重要作用。抗菌肽广泛存在于自然界的生物中,是动物先天性免疫系统中的重要组分。自1996年甲壳类抗菌肽首次在岸蟹(Carcinusmaenas)中报道以来,已有几种抗菌肽在蟹类血淋巴系统和生殖系统中被发现,它们是蟹类重要的免疫因子。因此,青蟹中新抗菌肽的揭示对于深入阐明其免疫机制及其在复杂的环境中健康生存具有重要的科学意义和应用价值 ...
单忠国
core  

Using customs data to understand overlooked trade in non‐CITES birds between Africa and Asia

open access: yesConservation Biology, EarlyView.
Abstract The international trade in live birds poses risks to animals, people, and biodiversity. To effectively mitigate these risks, decision‐makers require information on the volume, dynamics, and direction of trade. Despite Africa once being the largest exporter of birds by region, very little data exist on recent trade in live birds not listed on ...
Alisa Davies   +4 more
wiley   +1 more source

Æ E Æ Å – Reshaping the Role of Træna Fishermen

open access: yes, 2022
This diploma project aims to strengthen the viability of Træna’s community by proposing an ocean research facility in the archipelago, enabling the next generation of local fishermen to collect oceanographic data while working at sea. The idea of the fisherman as oceanographer is inextricably linked to the architectural program on the island of ...
openaire   +1 more source

中华绒螯蟹铁蛋白基因的克隆及表达分析 [PDF]

open access: yes, 2010
铁蛋白普遍存在于生物体内,具有铁离子解毒和储存等功能。在构建脂多糖刺激的中华绒螯蟹cDNA文库的基础上,获得了中华绒螯蟹铁蛋白基因EsFer的cDNA序列,其全长为1118bp,包含480bp的开放阅读框,推测编码的蛋白质为159氨基酸,其5′和3′端的非编码区分别为178bp和461bp,预测的分子量为18.3ku,理论等电点为5.81,在15~37aa处有一个跨膜螺旋。在5′非编码区核甘酸序列的135~162的位置有个特殊的结构,即铁反应元件(iron response element,IRE ...
付建平   +5 more
core  

Providing regular and frequent maps of losses and gains of farmland birds based on European monitoring data

open access: yesConservation Biology, EarlyView.
Abstract Knowledge of species distributions is essential for informing policies on nature conservation and restoration. However, updating them on a regular basis and doing so in a harmonized manner at the international level is difficult. The European Bird Census Council integrated national monitoring data covering 5 years to update farmland bird ...
Sergi Herrando   +54 more
wiley   +1 more source

载脂蛋白E PCR-RFLP分型的建立和评价

open access: yesZhongguo shiyan zhenduanxue, 1997
采用聚合酶链反应-限制性片段长度的多态性(polymerasechainreaction-restrictionfragmentlengthpolymorphism,PCR-RFLP)分析apoE的多态性。通过在引物5′端增加一段寡核苷酸,降低了扩增用二甲基亚砜(DMSO)的浓度,高效地扩增目标基因。运用限制性内切酶HhaI内切编码精氨酸的核苷酸顺序GCGC,不能切割编码半胱氨酸的核苷酸顺序GTGC。对含有编码有变异的112位和158位氨基酸的扩增产物进行酶解,用聚丙烯酰胺电泳(PAGE ...
陆元善, 吴文俊, 庄庆祺
doaj  

Seawater desalination by ICP on a new microfluidic chip [PDF]

open access: yes, 2015
全球淡水资源匮乏受到越来越多的国家的重视,特别是一些水资源短缺的国 家和地区。海水淡化被认为是获得淡水资源的最佳途径之一。现如今海水淡化方 法发展较为迅速,其中主流技术有反渗透法、电渗析法以及蒸馏法等。 虽然上述三种方法发展较早,技术相对成熟,但能耗大、运营成本高,需要 搭建大型配套设备且不便移动,不易全球推广,特别是在欠发达国家。在淡水资 源短缺急剧严重的今天,寻找一种低能耗、成本低、便携式、使用寿命长、易于 市场推广、适合家庭以及个人使用的新型海水淡化装置迫在眉睫,本文研究设计 ...
葛立凯
core  

The Cross‐Linguistic Transfer of Self‐Regulated Learning Strategy Use From L1 to L2: The Moderating Role of L2 Learning Motivation and Exposure

open access: yesInternational Journal of Applied Linguistics, EarlyView.
ABSTRACT Self‐regulated learning (SRL) as a key predictor of language learning has been widely studied, but few studies have examined it from a cross‐linguistic perspective. The present study investigated whether first language (L1) SRL strategy use transfers to second language (L2) SRL strategy use and the conditions under which this transfer occurs ...
Barry Bai, Xuan Zang, Gurpinder Lailli
wiley   +1 more source

载脂蛋白E PCR-RFLP分型的建立和评价 附视频

open access: yesZhongguo shiyan zhenduanxue, 1997
采用聚合酶链反应-限制性片段长度的多态性(polymerasechainreaction-restrictionfragmentlengthpolymorphism,PCR-RFLP)分析apoE的多态性。通过在引物5′端增加一段寡核苷酸,降低了扩增用二甲基亚砜(DMSO)的浓度,高效地扩增目标基因。运用限制性内切酶HhaI内切编码精氨酸的核苷酸顺序GCGC,不能切割编码半胱氨酸的核苷酸顺序GTGC。对含有编码有变异的112位和158位氨基酸的扩增产物进行酶解,用聚丙烯酰胺电泳(PAGE ...
陆元善, 吴文俊, 庄庆祺
doaj  

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