孟鲁司特抑制呼吸道合胞病毒感染人支气管上皮细胞所致炎症的药理机制
Zhongshan Daxue xuebao. Yixue kexue ban, 2017 【目的】探讨孟鲁司特抑制炎症过程中的药理机制。【方法】构建呼吸道合胞病毒感染人支气管上皮细胞 （16HBEC）炎症模型，分别用qPCR和Western-blot方法检测核因子NF-E2相关因子（Nrf2）、血红素加氧酶1（HO-1）、醌氧化还 原酶1（NQO-1）、谷胱甘肽硫转移酶（GST）的mRNA水平和蛋白水平，荧光探针DCFH-DA法检测细胞活性氧化物（ROS）的产 生。进一步通过合成Nrf2小干扰RNA（Nrf2 siRNA），下调Nrf2的表达后，探究炎症指标的变化情况。【结果 ...
江敏时 +3 more
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β-淀粉样肽对海马 CA1 区神经元的 短暂性外向 K+电流的影响
Zhongshan Daxue xuebao. Yixue kexue ban, 1998 目的: 为了在离子通道水平探讨β-淀粉样肽( β-AP)在 Alzheimer 病中的作用。方法: 用 7～ 21 d 大鼠,以链霉蛋 白酶 E 酶解加吸管吹打法制备海马 CA1区神经元; 利用全细胞膜片钳技术, 观察了β-AP25～ 35对急性分离海马 CA1 区锥体细 胞的短暂性 K+电流的影响。结果: β-AP25～ 35对短暂性 K+电流有明显抑制作用, 且呈明显浓度依赖性、时间依赖性和部分电 压依赖性。结论: β-AP 对 K+通道的影响在 Alzheimer 病的发生机制中具有重要作用 ...
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[Research progress of molecular imprinting technology in the field of disease diagnosis and therapy]. [PDF]
Se PuZhu R, Cai GP, Zheng HJ, Jia Q.
europepmc +1 more source
[The role of polyunsaturated fatty acid lipid peroxidation in ferroptosis after intracerebral hemorrhage: a review of mecha-nisms and therapeutic implications]. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue BanGuo M, Zhao G, Cai Z, Zhang Z, Zhou J.
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[Effect of dephosphorylation of tumor metastasis suppressor gene <i>LASS2</i> on vacuolar ATPase activity and invasiveness of prostate cancer]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanLiu Y +8 more
europepmc +1 more source
[Research progress on targeted delivery of proteasome inhibitor nanoparticles for the treatment of multiple myeloma]. [PDF]
Zhonghua Xue Ye Xue Za ZhiLin YX, Jing DH, Mi JQ.
europepmc +1 more source
[Role and mechanism of ubiquitin-specific protease 35 in ferroptosis of rheumatoid arthritis-fibroblast like synoviocytes]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanFeng L, Hong L, Chen Y, Cai X.
europepmc +1 more source
[The peptide toxin components and nucleotide metabolites in <i>Macrothele raveni</i> venom synergistically inhibit cancer cell proliferation by activating the pro-apoptotic pathways]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoXie T, Wang Y, Guo T, Yuan C.
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Zhongshan Daxue xuebao. Yixue kexue ban, 2015 摘 要: 【目的】 探讨SIRT6通过调节内皮一氧化氮合酶(eNOS)抑制苯肾上腺素(PE)诱导的新生大鼠心肌细胞肥大的作用及机制? 【方法】 在PE诱导的心肌细胞肥大模型中采用si-RNA干扰或腺病毒过表达的方法改变心肌细胞中SIRT6表达水平,并通过实时定量RT-PCR(real time PCR)?Western Blotting等方法检测心肌肥大基因心钠素(ANF)?脑尿钠肽(BNP)的表达?心肌细胞表面积,以及eNOS的表达及NO的生成?
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[Role and Mechanism of High Mobility Group Box 1 in Mediating Depression-Like Behaviors in Mice via Regulation of the 5-Hydroxytryptamine Receptor 7 Signaling Pathway]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanDU O, Duan S, Tang L, DU J.
europepmc +1 more source