Results 21 to 30 of about 1,895,360 (165)
Preliminary studies on transgenic human IFN-γ gene in tobacco(转人γIFN基因烟草的初步研究)
Zhejiang Daxue xuebao. Lixue ban, 2000 以TA克隆法构建中间载体PUCF1,再构建γ干扰素基因表达载体PBIF1。通过农杆菌转化导入烟草叶圆片外植体。经4轮梯度卡那霉素(Kanamycin)递增筛选，获得抗性植株。经PCR证实其中部分植株已将人γ干扰素基因片段整合到烟草基因组中，从而为成功地建立烟草植物反应器生产人γ干扰素基因奠定了基础。
YANGRong(杨蓉) +2 more
doaj +1 more source
Multi‐Functional S‐HA/ASTA NPs Coating on Biodegradable ZE21B Alloy for Cerebrovascular Applications
Rare Metals, Volume 45, Issue 2, February 2026.ABSTRACT Magnesium (Mg) and its alloys have attracted extensive attention of researchers in the field of cerebrovascular implants due to their good mechanical properties and biosafety. However, rapid degradation and delayed endothelialization pose challenges. To address these issues, this study introduces a multifunctional composite coating.
Aqeela Yasin +4 more
wiley +1 more source
四川大学学报. 自然科学版, 2013 油菜耐热基因(BnTR1)克隆自油菜,编码一种E3连接酶.为验证它的抗逆功能,本研究用携带BnTR1和新霉素磷酸转移酶(neomycin phosphotransferase,nptⅡ)基因的植物表达载体pCAMBIA2301-TR1的根癌农杆菌EHA105,对多年生黑麦草丛生芽进行遗传转化.经共培养和巴龙霉素筛选培养,获得了175株抗性植株.通过PCR和RT-PCR检测,获得62株转基因植株.结果表明,外源BnTR1基因已整合到转基因植株基因组中并在转录水平上表达.初步抗旱检测表明 ...
袁晓燕, 马欣荣, 李旭峰, 杨毅
doaj
Gongye shui chuli, 2023 研究Fenton法对污水处理厂剩余污泥中抗生素抗性基因（ARGs）和耐药菌（ARB）的去除效果，以明确Fenton对于降低污泥ARGs和ARB传播风险的有效性。采用宏基因组测定Fenton处理前后污泥，分析ARGs、移动遗传元件（MGEs）和微生物群落结构的变化。此外，以5种常见抗生素为基础，从Fenton处理后的污泥中筛选出ARB并推测其致病性。结果表明，经Fenton处理后污泥中ARGs明显下降，总去除率达到了67.36%。其中，特曲霉素类、春日霉素类、喹诺酮类和磷霉素类ARGs的去除量最高 ...
洪雯 +5 more
doaj
Effects of landscape fragmentation on floodplain fishes as revealed by species–habitat networks
Conservation Biology, Volume 40, Issue 1, February 2026.Abstract How species interact with habitat patches is influenced primarily by habitat configuration (e.g., connectivity) and species’ functional traits. As levels of fragmentation increase, identifying the intricate connections between these components is crucial for biodiversity conservation.
Chen Zhang +8 more
wiley +1 more source
SWEET1‐mediated glucose transport is crucial for energy availability in Arabidopsis
New Phytologist, Volume 249, Issue 4, Page 1816-1830, February 2026.Summary The stress‐responsive hormone abscisic acid (ABA) is known for its inhibitory effects on various physiological processes, including seed germination, often resulting in energy deprivation. Interestingly, ABA‐induced germination inhibition can be alleviated by exogenous glucose (Glc), mimicking a functional interplay between ABA and sugar ...
Xueyi Xue +3 more
wiley +1 more source
High nitrogen enrichment buffers plant photosynthesis against herbivory damage
Functional Ecology, Volume 40, Issue 1, Page 229-238, January 2026.Read the free Plain Language Summary for this article on the Journal blog. Abstract Herbivorous insects and nitrogen deposition are key drivers of ecosystem productivity under global change. However, their combined effects on plant photosynthesis, particularly during insect outbreaks, remain poorly understood in natural ecosystems.
Yongqiang Zhang +6 more
wiley +1 more source
Updated insights into the molecular pathogenesis of canine atopic dermatitis
Veterinary Dermatology, Volume 36, Issue 4, Page 375-384, August 2025.Abstract Atopic dermatitis (AD) is a common and chronic inflammatory skin disease with frequent relapses. The genomics revolution has greatly contributed and revolutionised our knowledge of human AD; understanding the molecular skin fingerprint of AD and associated pathogenic immune pathways has led to preclinical assessments of several novel ...
Frane Banovic
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 [目的]探讨大肠杆菌dinB基因在进化上的选择优势.[方法]应用竞争共培养和超表达的策略,测定共培养后含dinB基因的目的菌株和对照菌株的生长优势.第1组为含质粒pUC-dinB-rrnb的E.coli AB1157＋含质粒pUC-dinBR-rrnb的E.coli YG7207;第2组为含质粒pUC-dinB-rrnb的E..coli AB1157＋含质粒pUC-phoE-rrnb的E.coli YG7207;第3组为含质粒pUC-phoE-rrnb的E.coli AB1157＋含质粒pUC ...
席云, 肖刚, 吴坚, 林美玲
doaj
Functional Ecology, Volume 39, Issue 5, Page 1274-1287, May 2025.Read the free Plain Language Summary for this article on the Journal blog. Abstract Interactions between plants and soil microorganisms in the rhizosphere are vital for maintaining the nutrient cycle and stability of terrestrial ecosystems. Nitrogen, closely related to carbon (C) cycling and ecosystem productivity, undergoes transformation by soil ...
Ru Wang +9 more
wiley +1 more source