Zhongguo shiyan zhenduanxue, 2007 目的在大肠杆菌中以可溶性形式表达鼠源性α-1,3-半乳糖基转移酶的催化结构域（α1,3 GTcd;catalytic do-main ofα1,3GT）,利用其在人消化道肿瘤细胞表面构建α-gal表位（Galα1-3Gal-β1-4GlcNAc-R）,拟用来制备一种新的自体肿瘤疫苗。方法利用pET-15 b载体以可溶性形式表达鼠源性α1,3 GTcd,利用高效液相色谱阴离子交换柱检测酶的活性。通过其在人不同消化道肿瘤细胞表面构建α-gal表位,先与人血清孵育,FITC标记的羊抗人的IgG为第二抗体 ...
张睿, 迟宝荣, 朱学伟
doaj
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【目的】研究树突状细胞（Dc）在不同类型膀胱移行细胞癌及癌旁组织中的变化以及与肿瘤病理分级的关系，探讨恶性肿瘤免疫逃避的可能机制。【方法】将133例膀胱移行细胞癌病理标本按WHO标准进行病理分级，按1987年国际抗癌协会（UICC）标准进行临床分型（浅表和浸润），应用免疫组化法检测病理标本中Dc。【结果】133例标本中肿瘤Dc数目明显少于瘤旁组织（G1 16．85±1．1，G2 9．45±2．17，G3 2．99±1．19 vs G1 21．8±4．78，G2 21．71±4．72，G320．00±5 ...
丘少鹏 +3 more
doaj
[cGAS-STING agonist cGAMP enhances natural killer cell-mediated cytotoxicity against gastric cancer cells]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoWang Q +6 more
europepmc +1 more source
[Heterogeneity of tumor-associated macrophages and precision targeting strategies in hepatocellular carcinoma]. [PDF]
Zhonghua Gan Zang Bing Za ZhiHuang K, Zhang GQ.
europepmc +1 more source
[Clinical Progress and Prospects of mRNA Tumor Drugs]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanChen J +5 more
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 1998 目的: 研究小鼠 B7 修饰的肿瘤细胞疫苗的体内外抗肿瘤作用。 方法: 应用逆转录病毒载体, 将小鼠 B 7-1 和 B7-2 导入小鼠肝癌细胞 Hepal-6细胞株中, G418 筛选后获高表达 B7 分子的阳性细胞克隆,经丝裂霉素处理制备成肿瘤细胞 疫苗( TCV) ,观察 TCVB7体外刺激的脾细胞对不同肿瘤细胞系的抗瘤活性及对不同动物模型的体内免疫保护作用和免疫治疗 作用。结果: ①TCVB7体外刺激的脾细胞对亲本肝癌细胞和小鼠 NK 敏感的 Yac-1细胞的体外细胞毒作用明显增强; ②在免疫 ...
doaj
[Analysis of urine biomarkers in urothelial carcinoma based on untargeted metabolomics]. [PDF]
Se PuLiu S, Wang M, Wang W.
europepmc +1 more source
[CAR-T cell therapy bridging to allogeneic hematopoietic stem cell transplantation for diffuse large B-cell Richter syndrome: a case report and literature review]. [PDF]
Zhonghua Xue Ye Xue Za ZhiWang Y +5 more
europepmc +1 more source
[<i>Yiqi Jiedu</i> Formula inhibits proliferation, invasion and migration of nasopharyngeal carcinoma cells by inhibiting the AKT1/GLUT1 signaling pathway]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoXu H +8 more
europepmc +1 more source
[Research progress in liver cancer in 2025]. [PDF]
Zhonghua Gan Zang Bing Za ZhiMiao TG, Nan YM.
europepmc +1 more source