Results 11 to 20 of about 1,903,466 (129)
抗体のN末端特異的蛍光標識による新規抗原検出法の開発 [PDF]
, 2018 本研究では、既存のIgG抗体のN末端を特異的に蛍光標識することで、蛍光応答性抗体を作製する新規手法を開発した。弱酸性条件下で蛍光標識アルデヒドをIgG抗体と反応させることで、N末端特異的に蛍光基が付加され、得られたN末端蛍光標識抗体は、抗原の結合に伴って蛍光強度変化を示すことを明らかにした。また、FRETと蛍光消光解消を組み合わせることで、抗原を蛍光強度比の変化として検出可能な二重標識蛍光応答性抗体を合成すること、および、タグ化タンパク質の無細胞翻訳系での発現をリアルタイム検出することも可能であった ...
芳坂, 貴弘
core
Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT To address the demand for high‐temperature stability, broadband strong absorption, and multifunctional integration of electromagnetic wave absorbing materials in complex service environments, lightweight molybdenum disulfide (MoS2) decorated silicon carbide (SiC) nanofiber composite aerogels with a directional layered interconnected structure ...
Zhi Song +4 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2002 目的 检测类风湿性关节炎 (RA)患者血清抗Sa抗体 ,并探讨Sa抗体对RA的诊断意义。方法 Western blot方法。结果 抗Sa抗体诊断RA的敏感度为 42 .7% ,特异度为 1 0 0 % ,阳性确诊率为 1 0 0 % ,阴性确诊率为71 1 %。结论 血清抗Sa抗体对RA诊断的特异度和阳性确诊率均高于类风湿因子 (RF) ,因此具有临床实用价值。
王昶 +6 more
doaj
浙江大学学报. 农业与生命科学版Gibberellin-regulated protein (GASA) plays a significant role in plant growth, development and stress resistance. In this study, we used the industrial hemp (Cannabis sativa) cultivar ‘Longma 5’ as the material to identify and characterize the CsGASAs ...
YANG Sen(杨森) +8 more
doaj +1 more source
Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT Bone restoration is often hindered by the spatiotemporal dysregulation of immune and regenerative signals. Conventional bio‐inert implants, providing only passive support, fail to modulate this microenvironment, leading to infections and poor healing.
Zhizhong Sun +8 more
wiley +1 more source
Harnessing Trace Elements: Novel Radiosensitization Strategies for Enhanced Cancer Radiotherapy
Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT Radiotherapy (RT) is one of the core methods of comprehensive cancer treatment. Globally, more than 40% of cancer patients rely on radiotherapy to achieve tumor control, cure or palliative relief. However, radiation resistance, immunosuppression and normal tissue injury limit its clinical application.
Yuhuan Zhang +6 more
wiley +1 more source
抗环瓜氨酸肽抗体及相关自身抗体在类风湿性关节炎诊断中的应用评价
Zhongguo shiyan zhenduanxue, 2004 目的 评价抗环瓜氨酸肽 （CCP）抗体及其相关自身抗体对诊断类风湿性关节炎 （RA）的敏感性和特异性以及在临床上的意义。方法 收集 112例血清标本 ,包括RA 6 4例 ,系统性红斑狼疮 （SLE） 2 0例 ,骨关节炎 （OA） 16例 ,强直性脊柱炎 （AS） 12例 ,正常对照 30例 ,检测抗CCP、抗Sa、抗核周因子 （APF）和类风湿因子 （RF）。结果 4种自身抗体对诊断RA的敏感性和特异性分别为 :抗CCP 5 1 6 %和 97 9% ;抗Sa 4 6 9%和 10 0 ...
张建明, 丁玲华, 金跃, 葛志中
doaj
Rare Metals, Volume 45, Issue 6, June 2026.ABSTRACT Lung cancer is one of the most common and deadly malignancies worldwide. Although immune checkpoint inhibitors, including PD‐1/PD‐L1 blockers, have benefited a subset of patients, response rates remain limited, and recurrence is frequent because of the immunosuppressive tumor microenvironment (TME), characterized by hypoxia and dysregulated ...
Yi Ma +6 more
wiley +1 more source
, 2019 单克隆抗体因其与抗原结合具有高度特异性与强亲和力,已成为抗体药物研发的主要类型。但随着天然单克隆抗体的深入研究,它的诸多缺陷也浮出水面,如与抗原结合次数有限、带来非预期的抗体清除效应和抗原累积效应。人们不再局限于天然抗体的筛选,而是想通过改造提升抗体药物的药效。近年来,一类新型再循环抗体的问世,很好地解决了天然单克隆抗体发展的瓶颈。再循环抗体可以在胞外结合抗原,在细胞内与抗原解离,使抗体结合抗原次数最大化,减少抗原介导的抗体清除效应和抗体介导的抗原累积效应 ...
陈远志, 罗文新, 温灿
core +1 more source
一种抗对虾白斑综合症病毒(WSSV)线性抗原表位单链抗体的筛选
, 2008 对虾白斑综合症病毒(WSSV)是全世界对虾养殖业最主要的病原体之一,虽然对该病毒的研究已较为深入,但目前仍无有效的防治方法。本研究应用噬菌体展示技术,构建了抗变性WSSV的单链抗体噬菌体展示文库,分别以WSSV病毒粒子和原核表达的囊膜蛋白VP28为靶分子对该文库进行淘选。经过数轮淘选后,得到5个能特异识别WSSV的单链抗体,且首次获得了能特异识别WSSV线性抗原表位的单链抗体P75E8。并通过免疫胶体金电镜分析,对5种单链抗体对应在病毒粒子上的表位进行了定位 ...
戴和平 +4 more
core +1 more source