Results 31 to 40 of about 51,969 (188)

低共熔溶剂提取葵花籽饼中绿原酸 和总黄酮的工艺优化Optimization of extraction process of chlorogenic acid and total flavonoids from sunflower seed cake with deep eutectic solvent

open access: yesZhongguo youzhi
旨在促进葵花籽饼的高值化利用,采用低共熔溶剂(DES)提取葵花籽饼中绿原酸和总黄酮。以60%乙醇提取法为对照,绿原酸和总黄酮得率为评价指标,先从11种DES中确定最佳提取溶剂,在此基础上进一步考察提取时间、料液比、提取温度、DES含水量对绿原酸和总黄酮提取效果的影响,从而确定绿原酸和总黄酮的最优提取工艺。结果表明:综合考虑提取效果和成本,确定氯化胆碱-乳酸(物质的量比1∶ 2)为最佳提取溶剂;绿原酸的最优提取工艺条件为提取时间60 min、提取温度70 ℃、料液比1∶ 30和DES含水量30 ...
李志成,任曼曼,刘伟,梁少华,杨瑞楠,孙聪LI Zhicheng, REN Manman, LIU Wei, LIANG Shaohua, YANG Ruinan, SUN Cong
doaj   +1 more source

Road extraction from remote sensing images based on neighborhood total variation and edge detection(基于邻域总变分和边缘检测的遥感影像道路提取)

open access: yesZhejiang Daxue xuebao. Lixue ban, 2013
针对道路提取的同物异谱和同谱异物难题,提出了 一种基于邻域总变分和边缘检测的遥感影像道路提取方法,先对原始影像进行邻域总变分分割,提取出影像中具有均匀材质的地物,并采用Canny边缘检测方法获得原始影像的边缘数据;然后将边缘数据和分割结果融合,使均匀材质区域中的非道路地物和道路分离;最后采用多方向结构进一步提取狭长并具有一定宽度的道路.实验证明本文提出的道路提取方案能同时提取影像中光谱略有差异的道路,能有效剔除和道路黏连在一起的同谱异物地物,并能提取具有一定弯度的道路.
LAOXiao-min(劳小敏)   +5 more
doaj   +1 more source

Representation of obligate groundwater‐dwelling copepod diversity in European protected areas

open access: yesConservation Biology, EarlyView.
Abstract Groundwaters sustain diverse surface ecosystems and are populated by metazoan species, mostly invertebrates, that provide fundamental ecological functions and are often of prominent conservation value due to narrow endemism and high phylogenetic rarity.
Francesco Cerasoli   +7 more
wiley   +1 more source

Design and Research of Production Line of Continuous Cyclic Ultrasonic Extraction(连续循环超声波提取生产线的设计和研究) [PDF]

open access: yesShipin kexue jishu xuebao, 2014
The equipment structure and characteristics of ultrasonic extraction circulation were intro-duced based on the advantages and disadvantages of domestic ultrasonic extraction equipment.
LIU Yude(刘玉德)   +4 more
doaj   +1 more source

Using incentive payments to promote human–carnivore coexistence

open access: yesConservation Biology, EarlyView.
Abstract For many large carnivores, minimizing the financial burden they impose on local people is critical to their conservation. Incentive‐based programs that provide people with financial benefits for taking pro‐conservation actions or achieving conservation goals are a promising tool for promoting human–carnivore coexistence. Although the number of
Adam Pekor   +9 more
wiley   +1 more source

不同方法提取阴道加德纳菌细菌基因组DNA的比较

open access: yesZhongguo shiyan zhenduanxue, 2011
目的对阴道加德纳菌基因组DNA几种不同的提取方法进行比较,以利于细菌性阴道病的研究。方法采用几种不同方法提取阴道加德纳菌基因组DNA,对不同方法提取的细菌基因组DNA进行定量检测,以评价不同方法的提取效率。结果加德纳菌基因组DNA提取以改良法提取率较高,酚-氯仿抽提法、醋酸钾法次之,碱裂解煮沸法和溶菌酶煮沸法提取效率较差,加热煮沸法提取效率低。结论改良法提取GV基因组DNA效率较好,适用于临床应用。
姚虹, 吕治, 苏建荣
doaj  

厌氧条件在不同Fe( II) 浓度测定方法中必要性的比较研究 [PDF]

open access: yes, 2016
厌氧是目前Fe(II)测定方法中常选择的处理方式,但是不可避免会给实验操作带来不便。为了方便、准确地测定铁还原体系中的Fe(II)浓度,对比研究了厌氧条件对草酸—草酸铵和盐酸两种典型浸提方法测定Fe(II)浓度的影响。结果表明:对于草酸—草酸铵浸提法,厌氧条件是必须的,以防止提取液中的Fe(II)被氧化,而且样品浸提1 h 便可用于Fe(II)的测定;然而对于盐酸浸提法,厌氧条件却并不是必须的,对Fe(II)浓度测定基本不产生影响。因此,为简化实验操作或在没有厌氧条件的情况 ...
刘芳华   +1 more
core  

Constructed value of information with iterative scoring and parametric uncertainty to identify management‐relevant research priorities for a declining raptor species

open access: yesConservation Biology, EarlyView.
Abstract Constructed value of information (CVoI) is an expert elicitation decision‐analytic tool used to prioritize sources of uncertainty based on their potential to improve decision outcomes if resolved. Despite increased application of CVoI, the robustness of CVoI prioritization of sources of uncertainty relative to differences in expert elicitation
Kristin P. Davis   +7 more
wiley   +1 more source

高原地区大鼠耳蜗RNA提取条件优化

open access: yesZhongguo shiyan zhenduanxue, 2020
目的探讨在高原地区采用AMBION抽提试剂盒提取RNA的最佳条件。方法采用AMBION抽提试剂盒,对126份大鼠耳蜗(每份耳蜗分为2组,实验组和对照组)提取RNA,实验组采用AMBION抽提试剂盒优化条件提取RNA,对照组按照AMBION抽提试剂盒说明书提取RNA。结果实验组和对照组均能成功提取大鼠耳蜗组织RNA,总RNA的提取量分别为:0.896±0.348μg,0.536±0.404μg(P>0.05);实验组RNA浓度高于对照组,分别为:0.147±0.047μg/μl,0.072±0.022μg/
师晶晶   +5 more
doaj  

气相色谱外标法测定蛋黄卵磷脂(供注射用)中 7种脂肪酸含量Determination of 7 kinds of fatty acids in egg yolk lecithin(for injection) by GC with external standard method

open access: yesZhongguo youzhi, 2022
建立了适用于蛋黄卵磷脂(供注射用)中棕榈酸、棕榈油酸、硬脂酸、油酸、亚油酸、花生四烯酸、二十二碳六烯酸含量测定的分析方法。样品在具塞试管直接水浴加热甲酯化,正庚烷常温振荡提取后进气相色谱仪在起始柱温210 ℃下进行分析,利用外标法计算7种脂肪酸的含量。方法的专属性、精密度、准确度、耐用性考察均符合规定,在一定的质量浓度范围内,7种脂肪酸甲酯质量浓度与峰面积具有良好的线性关系,相关系数均大于0.999。该方法适用于蛋黄卵磷脂(供注射用)中7种脂肪酸含量的测定。 The method for the ...
温恺嘉,梁北梅,李咏华,林元亨,唐顺之,许文东 WEN Kaijia, LIANG Beimei, LI Yonghua, LIN Yuanheng, TANG Shunzhi, XU Wendong
doaj   +1 more source

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