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恶性实体瘤患者外周血细胞因子诱导的杀伤细胞增殖与杀瘤活性 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【目的】探讨恶性实体瘤患者外周血来源的细胞因子诱导的杀伤细胞的增殖能力、免疫表型及杀瘤活性。【方法】用rhlFN-1，rhlL-2，Anti-CD3mAb与恶性实体瘤患者外周血单个核细胞共孵育。分别在培养第4、7、10、13天进行细胞计数，通过流式细胞术检测细胞免疫表型及MTT法检测对K562细胞的杀伤活性。【结果】恶性实体瘤患者外周血单个核细胞与rhIFN-γ，rhIL-2，Anti-CD3mAb孵育4、7、10、13d，细胞数分别增加（2．5±1．6）、（11．9±3．5）、（22．3±7．8）和 ...
撒亚莲 +7 more
doaj +2 more sources
Zhongshan Daxue xuebao. Yixue kexue ban, 1992 采用单个细胞水平细胞毒方法研究比较了正常人外周血自然杀伤(NK)细胞对不同靶细胞结合与杀伤活性的差别。发现NK细胞对人胚胎成纤维细胞和低分化鼻咽癌(CNE-2)细胞的结合率明显高于对人成骨肉瘤细胞(OS-732)和PLC/PRF/5人肝癌细胞,结合并杀伤新鲜切除人肝癌细胞,而对同一病人的癌周肝细胞则无作用;在同一株CNE-2细胞中,NK多选择性地与核浆比例大的靶细胞结合。以上结果说明NK细胞在发挥自然细胞毒过程中具有一定的选择性,选择性地结合并杀伤分化差或幼稚的靶细胞。
doaj +2 more sources
CEA 重组痘苗病毒免疫巨噬细胞抑制CEA 阳性肿瘤的机制 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】探讨癌胚抗原重组痘苗病毒(CEA-rV)对癌胚抗原(CEA)阳性肿瘤的抑瘤机制。【方法】使用我室构建 的CEA-rV 腹腔接种C57BL/ 6 小鼠3 次, 6 周后, 取其腹腔巨噬细胞(MΥs)转输给携带CEA 阳性肿瘤的受体小鼠, 同时检测供 体MΥs 及受体脾细胞的体外细胞毒活性。【结果】供体MΥs 体外细胞毒活性无明显增加, 但体内却能明显抑制CEA 阳性肿 瘤的生长, 受体脾细胞对同一靶细胞的杀伤活性亦明显增强。【结论】经CEA-rV 接种处理的小鼠MΥs 可能通过其抗原提呈 ...
赵 青 +3 more
doaj +2 more sources
恶性疟原虫 FCC-1/HN 株 DNA 疫苗 在小鼠体内的免疫反应 [PDF]
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】探讨恶性疟原虫 DNA 疫苗在小鼠体内的免疫反应。【方法】将恶性疟原虫 FCC-1/HN 株重组质粒 pc DNA3-Pfs25 及 pcDNA3-EBA175/HRP Ⅱ经骨骼肌途径分别单独注射或两者混合注射免疫 BALB/c 小鼠, 观察免疫后不同时 间点血清中 IgG 抗体滴度、脾淋巴细胞增殖反应、CD4+/CD8+ T 细胞亚群比值和 NK 细胞杀伤活性的变化。【结果】pcDNA3-EBA175/HRPⅡ与 pcDNA3-Pfs25 分别单独肌肉注射或两者混合肌肉注射免疫小鼠后 ...
吕芳丽 +3 more
doaj +2 more sources
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】观察口腔鳞癌肿瘤浸润淋巴细胞和转移淋巴结淋巴细胞, 哪一种更适于作过继免疫治疗。【方法】从15 例口腔鳞癌原发灶及其中5 例转移灶中分离肿瘤浸润淋巴细胞和转移淋巴结淋巴细胞, 体外培养, 观察两种来源的淋巴细胞 增殖能力;用3H -TdR 4 h 释放法对5 例口腔鳞癌的肿瘤浸润淋巴细胞和转移淋巴结淋巴细胞对自体肿瘤杀伤活性进行比 较。【结果】转移淋巴结淋巴细胞较肿瘤浸润淋巴细胞扩增高峰延迟1 周, 转移淋巴结淋巴细胞培养5 周后仍显示强有力的 扩增潜力。3 ...
杨宏宇, 吴新中
doaj +2 more sources
Emergency management and the final frontier: Preparing local communities for falling space debris
Risk, Hazards &Crisis in Public Policy, Volume 14, Issue 3, Page 247-266, September 2023., 2023 Abstract Global dependence on satellite technologies, along with expanding space exploration, and commercial space travel have made rocket launches routine modern‐day events. As a result, there is an increasing probability of civilian casualty from rocket launch anomalies, or from falling space debris already in orbit.
Hans M. Louis‐Charles +3 more
wiley +1 more source
Effect of Life Review Therapy for Holocaust Survivors: A randomized controlled trial
Journal of Traumatic Stress, Volume 36, Issue 3, Page 628-641, June 2023., 2023 Abstract Despite the therapeutic needs of aging Holocaust survivors, no randomized controlled trial (RCT) of psychotherapy exists for this population, with very few on older adults in general. This RCT aimed to compare the efficacy of Life Review Therapy for Holocaust survivors (LRT‐HS) relative to a supportive control group. Holocaust survivors with a
Simon Forstmeier +6 more
wiley +1 more source
Veterinary Dermatology, Volume 27, Issue 5, Page 391-e98, October 2016., 2016 Background– Pseudomonas aeruginosa (PA) may cause suppurative otitis externa with severe inflammation and ulceration in dogs. Multidrug resistance is commonly reported for this organism, creating a difficult therapeutic challenge. Objective– The aim of this study was to evaluate the in vitro antimicrobial activity of a gel containing 0.5 µg/mL of ...
Giovanni Ghibaudo +6 more
wiley +1 more source
Leading research progress and prospect of antibody-drug conjugate in pancreatic cancer in 2022 [PDF]
, 2023 Pancreatic cancer is a type of malignant tumor with high incidence and low survival rate. It is urgent to develop novel and efficient therapeutic means for patients lacking surgical indications and patients with poor effects of radiotherapy and ...
ZENG Cheng, ZHANG Jian
core +1 more source
Research on T Lymphocyte Subsets and NK Cells Activity in Peripheral Blood of Hypopharyngeal Squamous Cell Carcinoma [PDF]
, 2017 目的:分析下咽鳞状细胞癌患者外周血T淋巴细胞亚群和NK细胞活性情况,旨在为相关研究工作提供参考资料。方法:择取2011年6月-2016年5月本院收治的95例下咽鳞状细胞癌病患为病例组,另取此期间内来本院接受健康检查的72例志愿者为正常组。使用流式细胞仪对病患开展相关检查,分析病例组与正常组的淋巴细胞亚群、NK活性情况以及不同临床分期的下咽癌病患外周血T淋巴细胞亚群以及NK细胞活性之间的关系。结果:与正常组相比,病例组的NK细胞、总B淋巴细胞、总T淋巴细胞、辅助/抑制T细胞、辅助T细胞偏低(P0.05 ...
刘存山 +7 more
core +1 more source