Zhongshan Daxue xuebao. Yixue kexue ban, 2015 摘 要: 【目的】 研究体外实验中,抗血小板药物替格瑞洛对原代小鼠树突状细胞(DC)摄取吞噬抗原以及促进T细胞增殖能力的影响? 【方法】 以RT-PCR?Western-Blotting方法测定细胞P2Y12受体基因和蛋白水平表达?实验分为空白对照组?单纯ADPβS刺激组?替格瑞洛+ADPβS组?以FITC标记葡聚糖(FITC-Dextran)作为荧光抗原检测DC吞噬抗原能力;将DC负载卵清蛋白(OVA),与OT-2转基因鼠脾脏T淋巴细胞共培养4 d,流式细胞仪检测荧光染料CFSE标记T细胞增殖情况? 【
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