【目的】 探讨脾细胞输注致敏对异基因供者骨髓细胞损伤的作用机制65377;【方法】 应用异基因脾细胞输注的BALB/c小鼠作为致敏模型,未致敏(正常)BALB/c小鼠作为对照,异基因供者骨髓细胞来源于C57BL/6小鼠65377;通过补体依赖细胞毒性反应(CDC)65380;细胞毒性免疫细胞杀伤作用65380;抗体依赖细胞介导的细胞毒性作用(ADCC)65380;粒-单核细胞集落培养(CFU-GM)等实验研究致敏血清及致敏免疫细胞对异基因骨髓细胞的影响65377;【结果】 与补体孵育30 min或1 ...
doaj
[Caplacizumab combined with therapeutic plasma exchange, glucocorticoids, and rituximab for refractory thrombotic thrombocytopenic purpura: a case report and literature review]. [PDF]
Wei QZ +5 more
europepmc +1 more source
[Impact of surgeon's dominant hand-side consistency with surgical approach on operational efficiency of unilateral biportal endoscopic lumbar discectomy: a prospective cohort study]. [PDF]
Li W, Gao M, Zhang L, Yu Q, Chen X.
europepmc +1 more source
[The influence of different collagenases on the analysis of immune cells in salivary glands of mice with chronic graft-versus-host disease]. [PDF]
Hu ZY, Song QX, Yang SJ, Wei J, Zhang X.
europepmc +1 more source
同一份血清中的HBVDNA与HCVRNA经热变性直接法一步裂解,先用针对HCV特异的外引物将HCVRNA逆转录为cDNA,然后采用HBV,HCV各自特异的2套引物进行PCR同步扩增。结果按预定大小,PCR产物出现2条带。分别为428bp,144bp。将产物转移至尼龙膜上,经α-32PdCTP标记HCV探针及地高辛素标记HBV探针进行重复杂交,证明144bp为HCV所特有,428bp为HBV特有。用该方法对30份单独检测证实HBV,HCV核酸均阳性血清测验,其结果完全吻合、该二联技术可明显缩短检测时间 ...
doaj
[<i>Huoxue Qingjie Ling</i> alleviates bile duct ligation-induced hepatic fibrosis in rats by regulating the Sirt1-autophagy signaling pathway]. [PDF]
Li C +6 more
europepmc +1 more source
[Gut-vascular axis in Kawasaki disease: short-chain fatty acid-mediated immune regulation and therapeutic targets]. [PDF]
Wang SD, Liu F.
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[Clinical research advances for small-molecule nucleic acid drugs in the treatment of chronic hepatitis B]. [PDF]
Wang WX +4 more
europepmc +1 more source
血清miR-124-3p、AXIN2表达水平对COPD合并2型糖尿病诊断的临床意义
目的探究血清微小核糖核酸-124-3p(miR-124-3p)、轴抑制蛋白2(AXIN2)表达水平对慢性阻塞性肺疾病(COPD)合并2型糖尿病诊断的临床意义。方法收集2022年8月至2023年8月中国人民解放军联勤保障部队第九八〇医院收治的COPD合并2型糖尿病患者106例为研究组,同期选择在本院确诊的单纯COPD患者106例为对照组。肺功能检测仪记录用力肺活量(FVC)、第1秒用力呼气量(FEV1)水平。采用实时荧光定量PCR(RT-qPCR)法检测血清中miR-124-3p、AXIN2水平 ...
张博智, 白维娟, 马惠
doaj
["Chongqing experiences" in treatment of bone infections]. [PDF]
Xie Z.
europepmc +1 more source

