Zhongshan Daxue xuebao. Yixue kexue ban, 1995 根据Ｂｅａｒ的ＥＢ病毒（ＥＢＶ）基因全序列，设计包括ＥＢＶ特异性ＤＮＡ酶全基因的一对引物，在引物上设计有一个ＳａｌⅠ切点，以便于克隆。选用蛋白酶Ｋ裂解的Ｒａｊｉ细胞ＤＮＡ为模板，扩增出一条１４６０ｂｐ的特异条带，纯比后经ＴａｑⅠ酶切形成１１９８ｂｐ和２６２ｂｐ的两个片段，证实该扩增片段即为ＥＢＶ特异性ＤＮＡ酶的全基因片段。以ＪＭ１０３为宿主菌，以高效表达质粒ＰＢＶ２２１为载体，按常规方法作酶切、连接、转化，最后在含氨中青霉素的培养基上挑取单菌落扩大培养，用快速法少量制备质粒ＤＮＡ。根据质粒电泳结果粗筛 ...
doaj
[Maximum limb reconstruction: concept and implementation strategies]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiZhu Y.
europepmc +1 more source
[Preoperative application effect of reverse traction technique in trimalleolar fracture-dislocation of ankle]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiChen M +5 more
europepmc +1 more source
基于三元Copula函数的三峡库区消落带岸坡动态失稳风险评估
Shuitu Baochi Xuebao目的为深化对复杂水文边界条件下岸坡动态失稳过程的风险评估，提出一种多因子联合概率—物理耦合分析框架。方法以降雨强度（I）、降雨历时（D）和库水位变动速率（R）为核心触发因子，基于三元Copula函数建立水文因子的联合分布，与非饱和渗流-稳定性有限元模型耦合，提出临界触发边界函数（CRPC），实现从水文情景到失稳响应的定量映射；考虑植被-排水协同效应，实现边坡失稳的联合概率评估。结果三元Clayton Copula（θ=10.213）在拟合优度与相关性检验中表现最佳，较好地刻画了水文因子的非线性依赖关系 ...
钟韵 +7 more
doaj
[Personalized reconstruction of post-traumatic calcaneal osteomyelitis with deformity using Ilizarov technique: a report of seven cases]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiZhang L +8 more
europepmc +1 more source
[Effectiveness comparison of one-hole split endoscopy and unilateral biportal endoscopy discectomy via the superior articular process approach for L <sub>5</sub>, S <sub>1</sub> far lateral lumbar disc herniation]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiLi X, Sun N, Sun Z, Li S, Qi G.
europepmc +1 more source
[Research Advances in ctDNA-MRD Monitoring in Local Treatment for Non-small Cell Lung Cancer]. [PDF]
Zhongguo Fei Ai Za ZhiLi Z, Wang Y, Wu Y, Guo Z.
europepmc +1 more source
[Analysis of epidemiological characteristics and spatial clustering of hepatitis C virus in China from 2018 to 2022]. [PDF]
Zhonghua Gan Zang Bing Za ZhiFan SF, Zhang JH, Duan ZH, Dai Z, He QY.
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野生动物学报朱鹮（Nipponia nippon）曾因栖息地破坏、环境污染和人为捕猎等因素，于1981年全球野生种群锐减至7只，濒临灭绝。经过40余年的保护管理，目前全球数量已恢复至1万余只，但其对应激刺激的高度敏感性和猝死风险仍对保护管理带来挑战。本研究基于对1990—2024年多个繁育基地事故报告的回顾性分析总结了其频繁猝死的环境诱因、行为表现和最终结局，并通过3例典型猝死案例对这类事件的病理生理学进行深入探究。在人工饲养条件下，朱鹮遭遇雷鸣、天敌干扰或环境变化时，易出现惊飞、撞击笼舍，导致外伤或突发性死亡 ...
邱国强 +10 more
doaj
[A special type of triple injury of superior shoulder suspensory complex-lateral segment floating clavicle]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiChen Y, Zhao H, Yang M, Xu S, Cai Y.
europepmc +1 more source