Identification of the CMV - resistance of transgenic tomato plants [PDF]
, 1995 上海农业学报基因番茄对CMV-cp病毒抗性鉴定杨荣昌徐鹤林余文贵陆春贵 龙明生 (江苏省农业科学院蔬菜研究所,南京。14)刘春清潘乃褪 陈章良(北京大学植物基 因工程国家重点实验室,北京1。。871)摘 要 苗期人工接种,对转化CMV一叩基因 的番茄品种8805RI~R4代进行接种CMV病毒试验。结果表明:黄瓜花叶病毒外壳蛋白基因在番茄中的表达,对抑制CMV病毒病症状的表现及延迟其发展均起到一定作用。 8805R3代对CMV的抗性明显高于R:代,R;代抗性已基本稳定。当高浓度的CMV病毒侵染R ...
Chen, Z +7 more
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Comprehensive quality detection method for tomatoes combining machine vision and spectral techniques [PDF]
ObjectiveTo realize rapid and accurate measurement of both internal and external quality of tomatoes, and improve the efficiency and quality of tomato grading.MethodsBased on machine vision and spectroscopy technology, proposed a tomato comprehensive ...
GUO Dechao +4 more
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Shuitu Baochi Xuebao, 2008 通过调查不同棚龄大棚土壤硝态氮含量的变化,研究了杨凌大棚蔬菜生产中的土壤硝态氮累积特性,结果显示:大棚蔬菜土壤硝态氮含量显著高于露天菜地和拱棚土壤,反映出过高的氮肥投入,各棚龄土壤均表现出明显的硝态氮表土累积。随着棚龄的增加土壤硝态氮及总盐含量呈增加趋势,土壤pH值则表现为逐渐下降,不合理的施肥将导致短龄大棚土壤硝态氮及总盐含量急剧上升而超过长龄大棚土壤;在番茄生长周期内随生育期延长硝态氮在土壤中累积量逐渐增大,但总盐含量变化并不明显。硝态氮在土壤中的迁移,导致土壤底层硝态氮也大量累积。
魏迎春, 李新平, 刘刚, 朱辉娟
doaj
Study on Tomato Transformation with Human Keratinocyte Growth Factor-1 [PDF]
, 2011 人角质细胞生长因子在组织的损伤修复中起着重要的作用.以番茄子叶为外植体,通过根癌农杆菌介导法,将人kgf-1导入番茄中,共获得12株独立的抗性转化植株,PCr和dnA斑点杂交结果表明:kgf-1基因整合入番茄基因组中,为获得植物源表达的kgf-1蛋白打下了基础.Keratinocyte growth factor plays an important role in the tissue damage repair.Using sterile tomato cotyledons as explants ...
张国广, 张辉煌, 邹金美, 陈亮
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Analysis of Changes in Flavor Substances of Tomato Fruits in Different Ripening Stages by Headspace-Gas Chromatography-Ion Mobility Spectrometry [PDF]
High performance liquid chromatography (HPLC) and headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) were employed to analyze the changes in volatile metabolites in wild-type Ailsa Craig tomato fruits in four ripening stages (mature green,
FU Xiaohui, SHI Lu, SUN Qingzhen, DONG Xiaoxing, PIAO Fengzhi, DU Nanshan
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[Development of a widely-targeted metabolomics method based on gas chromatography-mass spectrometry]. [PDF]
Se Pu, 2023 Wang YT, Yang Y, Sun XL, Ji J.
europepmc +1 more source
Oral opening training increases oral opening in patients with oral submucous fibrosis. [PDF]
Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2021 Chen J, Wu Y, Peng J, Ma L.
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Studies on Construction of Plant Expression Vector of Recombinant LBM2eHBc+ Gene and Tomato Transformation [PDF]
, 2010 禽流感病毒一直是家禽和家畜健康养殖的巨大威胁,甚至威胁着人类的健康,人们也一直在研究各种形式的疫苗来应对禽流感病毒的威胁.番茄作为生物反应器应用于动物疫苗生产具有独特的优点,本研究优化了番茄子叶的再生体系,同时构建了lbM2EHbC+融合基因的植物表达载体并对番茄进行了遗传转化.结果表明在玉米素(zT)浓度为0.5 Mg/l时番茄子叶外植体的芽再生率达到93.33%;测序结果证明植物表达载体PbI121-lbM2EHbC+构建成功;利用农杆菌介导的转化方法获得再生抗性苗34株,经PCr检测,阳性率为75.
张国广, 张辉煌, 董美, 陈亮
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[Research advances on the development and application of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein system]. [PDF]
Zhonghua Shao Shang Za Zhi, 2021 Tan JJ, Peng YZ, Huang GT.
europepmc +1 more source
Study on the effects of an avirulent strain of Ralstonia solanacearum-GFP on suppressing Bacterial Wilt Disease in the tomato [PDF]
, 2007 细菌性青枯病是一种由青枯雷尔氏菌(Ralstoniasolanacearum)引起的植物毁灭性病害，广泛分布于热带、亚热带及温带地区。青枯雷尔氏菌寄主广泛，可以侵染44个科数百种植物，对农业造成了重大的经济损失。青枯病的生物防治越来越引起大家的重视。本文观察比较了青枯雷尔氏菌无致病力菌株Rs-L.1.3-020626-1A转GFP基因前后的菌落形态和单细胞形态；研究了无致病力菌株GFP-Rs-L.1.3-020626-1A对番茄组培苗的侵染特性，筛选出了最适侵染体系；研究了GFP-Rs-L.1.3 ...
杨淑佳
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