【目的】 观察缬沙坦对泡沫细胞血管内皮生长因子(VEGF)mRNA表达与蛋白分泌的影响?【方法】 以氧化型低密度脂蛋白刺激体外培养的人单核细胞株U937细胞,建立U937泡沫细胞模型?采用不同浓度的缬沙坦(0.01μmol/L、0.1 μmol/L、1 μmol/ L)进行干预?RT-PCR检测泡沫细胞VEGF mRNA表达,ELISA检测泡沫细胞VEGF蛋白的分泌。【结果】 U937泡沫细胞VEGF mRNA表达(2.371 ± 0.253)和VEGF蛋白分(1804.18 ± 177.59 pg ...
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